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Series GSE124848 Query DataSets for GSE124848
Status Public on Jan 10, 2019
Title Directed endocytosis mediates immune communication between an epithelial commensal microbe and the host
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Commensal bacteria influence host physiology, including immune responses, without invading host tissues. We show that proteins from segmented filamentous bacteria (SFB), which are immunomodulatory commensal microbes, are transferred into intestinal epithelial cells by adhesion-directed endocytosis that is distinct from the clathrin-dependent endocytosis of invasive pathogens. SFB transfer microbial cell wall-associated proteins, including an antigen that stimulates mucosal Th17 cell differentiation, into the cytosol of epithelial cells. Removal of CDC42 activity in vivo led to disruption of endocytosis induced by SFB, decreased epithelial antigen acquisition with consequent loss of immune modulation by SFB-specific CD4 T cells and mucosal Th17 cells. Our findings indicate direct communication between a resident gut microbe and the host and show that intestinal epithelial cells acquire antigens from commensal bacteria for generation of T-cell responses to the resident microbiota.
Overall design Intestinal epithelial cells from WT and IECΔCDC42 mice were isolated to performed RNAseq.
Contributor(s) Ivanov I, Araujo L
Citation(s) 30846568
Submission date Jan 09, 2019
Last update date Apr 15, 2019
Contact name Leandro Pires Araujo
Phone 6466103421
Organization name Columbia University
Department Microbiology and Immunology
Lab Ivanov Lab
Street address 701 W 168th St, 9th floor
City New York
State/province New York
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (10)
GSM3557103 WT + SFB (IL001)
GSM3557104 IECΔCDC42 +SFB (IL002)
GSM3557105 IECΔCDC42 +SFB (IL003)
BioProject PRJNA513988
SRA SRP178075

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Supplementary file Size Download File type/resource
GSE124848_Processed_data_files_DESeq2_Kallisto.xlsx 5.8 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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