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Series GSE124796 Query DataSets for GSE124796
Status Public on Feb 11, 2019
Title CD69+ memory T lymphocytes of the bone marrow and spleen express the signature transcripts of tissue-resident memory T lymphocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Expression profiling by array
Summary To understand tissue resident features of memory CD4+ and CD8+ T lymphocytes of the bone marrow and/or spleen according to expressing or not the tissue retention marker CD69, we performed whole transcriptome profiling of ex vivo antigen-specific CD69+ and CD69- memory CD4+ T cells isolated from bone marrow and spleen, and ex vivo CD69+ and CD69- memory CD8+ T cells isolated from bone marrow.
Overall design For CD4 analysis, 8-week-old C57BL/6 mice were primed at day 0 with LCMV.GP61-80-NP-MSA + poly(I:C) and immunized again at day 14 with LCMV.GP61-80 + poly(I:C). Sixty days after the last immunization mice were sacrificed and LCMV.GP66–77-specific CD69+ and CD69- memory CD4+ T cells were isolated from BM and spleen by FACS. The whole transcriptome profiling of purified cells was performed by RNA-seq. For CD8 analysis, single cell suspensions were prepared from bone marrow of 8-week old untreated C57BL/6 male mice. CD8+ T cells were pre-enriched with anti-CD8a microbeads and the CD69+ and CD69- subpopulations of memory CD8+ T cells were then FACS purified. The whole transcriptome profiling of sorted cells was analyzed by microarray analysis.
Citation(s) 30673129
Submission date Jan 08, 2019
Last update date Jun 24, 2019
Contact name Pawel Durek
Organization name Deutsches Rheuma-Forschungszentrum
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platforms (2)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM3554173 BM_CD69pos_1
GSM3554174 BM_CD69neg_1
GSM3554175 BM_CD69pos_2
This SuperSeries is composed of the following SubSeries:
GSE124794 Whole-transcriptome profiling of LCMV.GP66-77 specific CD4 T cells isolated from bone marrow (BM) and spleen, 60 days after last immunization [RNA-seq]
GSE124795 Whole-transcriptome profiling of murine steady state CD69+ and CD69- memory CD8+ T cells from the bone marrow [expression microarray]
BioProject PRJNA513497

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Supplementary file Size Download File type/resource
GSE124796_RAW.tar 22.2 Mb (http)(custom) TAR (of CEL, TXT)
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