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Series GSE124718 Query DataSets for GSE124718
Status Public on Jul 05, 2019
Title Resetting histone modifications during human parental-to-zygotic transition
Organisms Homo sapiens; Mus musculus
Experiment type Other
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Histone modifications regulate gene expression and development. To address how they are reprogrammed in human early development, we investigated key histone marks in human oocytes and early embryos. Unlike that in mouse, the permissive mark H3K4me3 largely exhibits canonical patterns at promoters in human oocytes. After fertilization, pre-zygotic genome activation (ZGA) embryos acquire permissive chromatin and widespread H3K4me3 in CpG-rich regulatory regions. By contrast, the repressive mark H3K27me3 undergoes global depletion. CpG-rich regulatory regions then resolve to either active or repressed states upon ZGA, followed by subsequent restoration of H3K27me3 at developmental genes. Finally, through combining chromatin and transcriptome maps, we revealed transcription circuitry and asymmetric H3K27me3 patterning during early lineage specification. Collectively, our data unveil a priming phase connecting human parental-to-zygotic epigenetic transition.
 
Overall design We investigated the dynamics of two key histone marks H3K4me3, H3K27me3 in human oocytes and pre-implantation embryos, and H3K27ac in post-ZGA embryos using CUT&RUN.
 
Contributor(s) Xia W, Xu J, Yu G, Yao G, Sun Y, Xie W
Citation(s) 31273069
Submission date Jan 07, 2019
Last update date Aug 03, 2019
Contact name Wei Xie
E-mail(s) xiewei121@tsinghua.edu.cn
Organization name Tsinghua University
Department School of Life Science
Lab Wei Xie
Street address Qinghuayuan
City Beijing
State/province Beijing
ZIP/Postal code 100084
Country China
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (42)
GSM3543849 hs_GV_H3K4me3_rep1
GSM3543850 hs_GV_H3K4me3_rep2
GSM3543851 hs_GV_H3K27me3_rep1
Relations
BioProject PRJNA513257
SRA SRP176396

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124718_2cell_3PN_H3K27me3.broadPeak.gz 868.3 Kb (ftp)(http) BROADPEAK
GSE124718_4cell_2PN_H3K4me3.broadPeak.gz 3.5 Mb (ftp)(http) BROADPEAK
GSE124718_4cell_3PN_H3K27me3.broadPeak.gz 41.8 Kb (ftp)(http) BROADPEAK
GSE124718_4cell_3PN_H3K4me3.broadPeak.gz 3.3 Mb (ftp)(http) BROADPEAK
GSE124718_8cell_2PN_H3K27me3.broadPeak.gz 6.9 Kb (ftp)(http) BROADPEAK
GSE124718_8cell_2PN_H3K4me3.narrowPeak.gz 687.4 Kb (ftp)(http) NARROWPEAK
GSE124718_8cell_3PN_H3K27ac.broadPeak.gz 4.0 Mb (ftp)(http) BROADPEAK
GSE124718_8cell_3PN_H3K27me3.broadPeak.gz 108.0 Kb (ftp)(http) BROADPEAK
GSE124718_8cell_3PN_H3K4me3.narrowPeak.gz 1.5 Mb (ftp)(http) NARROWPEAK
GSE124718_GV_ATAC.narrowPeak.gz 514.8 Kb (ftp)(http) NARROWPEAK
GSE124718_GV_H3K27me3.broadPeak.gz 2.0 Mb (ftp)(http) BROADPEAK
GSE124718_GV_H3K4me3.narrowPeak.gz 1.2 Mb (ftp)(http) NARROWPEAK
GSE124718_ICM_2PN_H3K27ac.broadPeak.gz 3.8 Mb (ftp)(http) BROADPEAK
GSE124718_ICM_H3K27me3.broadPeak.gz 2.6 Mb (ftp)(http) BROADPEAK
GSE124718_ICM_H3K4me3.narrowPeak.gz 503.4 Kb (ftp)(http) NARROWPEAK
GSE124718_MI_H3K4me3.narrowPeak.gz 958.1 Kb (ftp)(http) NARROWPEAK
GSE124718_RAW.tar 2.0 Gb (http)(custom) TAR (of BEDGRAPH, TXT)
GSE124718_TE_2PN_H3K27me3.broadPeak.gz 1.7 Mb (ftp)(http) BROADPEAK
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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