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Series GSE124660 Query DataSets for GSE124660
Status Public on Jul 01, 2019
Title mRNA expression profile of mouse CD4 T cells (treated vs. untreated with PM2.5 suspension)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: PM2.5 can impact mouse TH17 cell differentitation. To Investigator the genes altered by PM2.5 during TH17 differentiation, mRNA-seq was performed.
Methods: Naïve T cells were isolated from mouse spleen by magnetic beads. These naïve T cells were stimulated by CD3/CD28 antibodies and cultured under TH17 polarization conditions for 4 days before harvested. For PM2.5 group, 100ug/ml of PM2.5 suspensions were added to culture medium 6 hours after stimulation. The total RNA was extracted using the TRIzol reagent (Invitrogen). The library construction and sequencing was performed by Illumina Xten at Shanghai Biotechnology Corporation.
Overall design PM2.5 treated group was compared to control group. Each group has 3 samples.
Contributor(s) Zhou Y, Sun L
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Submission date Jan 04, 2019
Last update date Jul 01, 2019
Contact name Sun Licheng
Organization name Fudan University
Street address No. 399, wanyuan road
City Shanghai
ZIP/Postal code 201102
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3538849 A1
GSM3538850 A2
GSM3538851 A3
BioProject PRJNA513007
SRA SRP175258

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Supplementary file Size Download File type/resource
GSE124660_gene_expression.txt.gz 2.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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