GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE124495 Query DataSets for GSE124495
Status Public on Oct 17, 2019
Title NPM and NPM-MLF1 interact with chromatin remodeling complexes and influence their recruitment to specific genes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Nucleophosmin (NPM1) is either frequently mutated or subjected to chromosomal translocation in acute myeloid leukemia (AML). NPM protein is primarily located in the nucleus, but the recurrent NPMc+ mutation is characterized by cytoplasmic localization and leukemogenic properties. Similarly, the NPM-MLF1 translocation product favors the partial cytoplasmic retention of NPM. Regardless of their common cellular distribution, NPM-MLF1 malignancies engender different effects on hematopoiesis compared to NPMc+ counterparts, highlighting possible aberrant nuclear function(s) of NPM in NPMc+ AML. We performed a proteomics analysis and found that NPM and NPM-MLF1 interact with chromatin remodeling complexes of the ISWI family, as well as with NuRD and P/BAF complexes. Accordingly, NPM and NPM-MLF1 are recruited to transcriptionally active or repressed genes along with NuRD and P/BAF elements. Although the overall gene expression program in NPM knockdown cells is similar to that resulting from NPMc+ and is consistent with loss of nuclear function of NPM, NPM-MLF1 expression differentially altered gene transcription regulated by NPM. The abnormal gene regulation imposed by NPM-MLF1 is characterized by enhanced recruitment of NuRD to gene regulatory regions. Thus, different mechanisms orchestrate the deregulation of NPM function in NPMc+- versus NPM1-MLF1-associated leukemia.
Overall design Gene expression profil was determinated by Ion Ampli Sequencing in K562 sh Scramble (contrôle) and K562 shNPM cells, in triplicate.
Contributor(s) Milot E
Citation(s) 31675375
Submission date Dec 29, 2018
Last update date Dec 05, 2019
Contact name Eric Milot
Phone 514 252 3551
Organization name HMR research center/Université de Montréal
Department Medicine
Lab JADS 2nd floor
Street address 5415 boul. l'Assomption
City Montréal
State/province Québec
ZIP/Postal code H1T 2M4
Country Canada
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (6)
GSM3535282 sh Scramble n1
GSM3535283 sh Scramble n2
GSM3535284 sh Scramble n3
BioProject PRJNA512203
SRA SRP174885

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124495_RAW.tar 10.3 Mb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap