GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE124494 Query DataSets for GSE124494
Status Public on Aug 08, 2019
Title Single-cell survey of human lymphatics unveils marked endothelial cell heterogeneity and mechanisms of homing for neutrophils
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Lymphatic vessels form a critical component in the regulation of human health and disease. While their functional significance is increasingly being recognized, the comprehensive heterogeneity of lymphatics remains uncharacterized. Here, we report the profiling of 33,000 lymphatic endothelial cells (LECs) in human lymph nodes (LNs) by single-cell RNA sequencing. Unbiased clustering revealed six major types of human LECs. LECs lining the sub-capsular sinus (SCS) of LNs abundantly expressed neutrophil chemoattractants, whereas LECs lining the medullary sinus (MS) expressed a C-type lectin CD209. Binding of a carbohydrate Lewis X (CD15) to CD209 mediated neutrophil binding to the MS. The neutrophil-selective homing by MS LECs may retain neutrophils in the LN medulla and allow lymph-borne pathogens to clear, preventing their spread through LNs in humans. Our study provides a comprehensive characterization of LEC heterogeneity and unveils a previously undefined role for medullary LECs in human immunity.
Overall design Droplet-based single-cell RNA sequencing (scRNAseq) of human CD45- CD31+ PDPN+ cells from the axillary LNs (AXLNs) and head and neck LNs (HNLNs). ScRNAseq was performed using 10X Genomics Single Cell 3' solution, version 2 according to manufacture's instructions. Libraries were sequenced on HiSeq3000.
Contributor(s) Takeda A, Jalkanen S
Citation(s) 31402260
Submission date Dec 29, 2018
Last update date Aug 15, 2019
Contact name Akira Takeda
Organization name University of Turku
Department Institute of Biomedicine
Street address Tykistökatu 6A
City Turku
State/province South-West Finland
ZIP/Postal code 20520
Country Finland
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM3535276 AXLN1
GSM3535277 AXLN2
GSM3535278 AXLN3
BioProject PRJNA512202
SRA SRP175077

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124494_RAW.tar 189.2 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap