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Series GSE124360 Query DataSets for GSE124360
Status Public on Dec 27, 2018
Title RAW246.1 cells activation by internalized synthetic miR-122
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hepatic injury is often accompanied by pulmonary inflammation and tissue damage, but the underneath mechanism is not fully elucidated. Here we identify hepatic miR-122 as a culprit of pulmonary inflammation induced by various liver injuries. Analyses of acute and chronic liver injury mouse models confirm that liver dysfunction can cause pulmonary inflammation and tissue damage. Injured livers release large amounts of miR-122 in a microvesicle-independent manner into the circulation compared to normal livers. Circulating miR-122 is then preferentially transported to mouse lungs and taken up by alveolar macrophages, in which it binds toll-like receptor 7 (TLR7) and activates inflammatory responses. Depleting plasma miR-122 largely abolishes liver injury-induced pulmonary inflammation and tissue damage. Furthermore, alveolar macrophage activation by miR-122 is blocked by mutating the TLR7-binding UG-rich sequence on miR-122 or knocking out macrophage TLR7. Our findings reveal a novel causative role of hepatic miR-122 in liver injury-induced pulmonary dysfunction.
Overall design To explore the molecular basis underlying the effect of hepatic miR-122 on alveolar macrophage inflammatory activation, we performed transcription profiling in macrophages treated with or without miR-122.
Contributor(s) Wang Y, Zen K
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Submission date Dec 26, 2018
Last update date Dec 28, 2018
Contact name Yanbo Wang
Organization name Nanjing University
Street address 163 Xianlin Road
City Nanjing
ZIP/Postal code 210023
Country China
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (2)
GSM3530398 Mock: Cells_PBS_rep1
GSM3530399 miR-122: Cells_without miR-122_rep1
BioProject PRJNA511852

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124360_RAW.tar 4.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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