NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE124358 Query DataSets for GSE124358
Status Public on Feb 01, 2020
Title Analysis of DNA Methylation in Sperm, egg cell, fertilized zygote and preimplantation embryonic cells
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary 10% of methylation sites in CpG islands. In this study, the genome-wide CpG islands of human sperm, oocyte and pre-implantation embryos were analyzed using the almost complete coverage of promoters and CpG islands (most methylation-producing regions) methylation microarray method (MeDIP-Chip). Dynamic changes in methylation of sub-regions to understand the dynamic pattern of CpG island and promoter methylation, possible regulatory mechanisms of this methylation dynamic change, and function during early embryonic development.
 
Overall design This study showed that the methylation of whole-genome CpG islands in human pre-implantation embryos showed a unique dynamic pattern: after fertilization, the prokaryotic nucleus began to demethylate, and the 4cell phase of the whole genome CpG island had the lowest degree of methylation, and then gradually re-established. The methylation pattern was established; the ICM methylation level in the blastocyst stage was close to that of the oocyte, and the methylation level of TE was between the oocyte and the sperm; while the human and mammalian preimplantation embryos were in the whole genome. The lowest level of the level is in the ICM stage. There are three dramatic changes in the methylation of CpG islands in the pre-embryo of the genome at different developmental stages. The main stage of methylation erasure of CpG islands is 4cell phase, while the whole genome-wide erasure is in 2cell phase.
 
Contributor(s) Huang Y, Li L
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 26, 2018
Last update date Feb 03, 2020
Contact name yuling huang
E-mail(s) gzgyhyl@163.com
Phone +86 13922213365
Organization name Center for Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University
Street address No. 250, Changgang East Road, Haizhu District, Guangzhou
City guangzhou
ZIP/Postal code 510150
Country China
 
Platforms (1)
GPL16378 NimbleGen Human DNA Methylation 3x720K CpG Island Plus RefSeq Promoter Array [100718_HG18_CpG_Refseq_Prom_MeDIP]
Samples (8)
GSM3530386 A
GSM3530387 B
GSM3530388 C
Relations
BioProject PRJNA511826

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124358_Data_Analysis_Result.xlsx.gz 48.9 Mb (ftp)(http) XLSX
GSE124358_RAW.tar 364.9 Mb (http)(custom) TAR (of GFF, PAIR)
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap