Given that celastrol?s leptin-sensitizing effect requires high levels of circulating leptin, but lean mice have low levels of circulating leptin so that celastrol has no effect on lean mice. Analysis celastrol-induced hypothalamic gene expression profile change in lean mice will also be serving as negative control for DIO mice analysis.
Overall design
lean mice were injected with celastrol or DMSO as vehicle control for 6h, 1day or 4 days and then the RNA was extracted from hypothalamus for microarray
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