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Series GSE124348 Query DataSets for GSE124348
Status Public on Dec 26, 2018
Title STAT3 secretion defect results in development abnormally
Organism Mus musculus
Experiment type Expression profiling by array
Summary Total RNA from embryos (12 days) of STAT3mut/mut, STAT3mut/wt and STAT3wt/wt mice which come from the same womb were extracted using TRIzol reagent (Invitrogen) following the manufacturer’s protocols. RNA quantity, quality and integrity were analyzed with NanoDrop ND-1000 and standard denaturing agarose gel electrophoresis. Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol of Agilent Technology. The Whole Mouse Genome Oligo Microarray (Agilent 4*44k) represents all known genes and transcripts in the mouse genome (Kyoto Encyclopedia of Genes and Genomes, KEGG). All the microarray experiments were performed by Kangchen Biotechnology Limited (Shanghai, China).
Overall design Gene expression in embryos fromSTAT3-K177K180R mutant mice (wild type, heterozygous, and homozygous) were used for gene expression. Two independent experiments were performed at each group.
Contributor(s) Xu L
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Submission date Dec 25, 2018
Last update date Dec 27, 2018
Contact name Li Xu
Phone 13003691133
Organization name ZJTCMU
Street address BinWen Street 358#
City HangZhou City
State/province ZheJiang Province
ZIP/Postal code 310053
Country China
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (6)
GSM3530262 Embryo from homozygous mutant (STAT3-K177K180R) mice 1
GSM3530263 Embryo from homozygous mutant (STAT3-K177K180R) mice 2
GSM3530264 Embryo from heterozygous mutant (STAT3-K177K180R) mice 1
BioProject PRJNA511761

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124348_RAW.tar 12.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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