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Series GSE124335 Query DataSets for GSE124335
Status Public on Feb 13, 2019
Title Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Microglia play critical roles in neural development and homeostasis. They are also implicated in neurodegenerative and neuroinflammatory diseases of the central nervous system (CNS). However, little is known about the presence of spatially and temporally restricted subclasses of microglia during CNS development and disease. Here, we combined massively parallel single-cell analysis, single-molecule FISH, advanced immunohistochemistry and computational modelling to comprehensively characterize novel microglia subclasses, which were transcriptionally different from perivascular macrophages, in up to six different CNS regions during development and diseases. Single-cell analysis revealed specific time- and region-dependent microglia subtypes during homeostasis. In contrast, demyelinating and neurodegenerative diseases evoked context-dependent microglia subtypes with distinct molecular hallmarks and diverse cellular kinetics. Finally, diverse microglia subsets were also identified in normal and diseased human brains. Our data provide new insights into the CNS endogenous immune system during development, health and perturbations.
 
Overall design CD45+ cells isolated from healthy and MS-affected human brains were FACS-sorted in 384-well plates and used for scRNAseq. The patients were aged between 22 and 25 years. Data comprises 5 healthy and 5 MS patients. CEL-Seq2 protocol was used for single cell sequencing (Hashimshony et al. 2016).
 
Contributor(s) Masuda T, Sankowski R, Staszewski O, Sagar -, Gruen D, Prinz M
Citation(s) 30760929
Submission date Dec 24, 2018
Last update date Feb 16, 2019
Contact name Sagar -
E-mail(s) sagar@uniklinik-freiburg.de
Organization name University Medical Center Freiburg
Department Department of Internal Medicine II
Lab Sagar
Street address Hugstetter Stra├če 55
City Freiburg
ZIP/Postal code 79106
Country Germany
 
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (32)
GSM3529822 MS_case1_3.coutt.csv
GSM3529823 MS_case1_4.coutt.csv
GSM3529824 MS_case1_Pl1_1_9.coutt.csv
Relations
BioProject PRJNA511668
SRA SRP174409

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124335_RAW.tar 8.8 Mb (http)(custom) TAR (of CSV)
GSE124335_celseq_barcodes.192.txt.gz 910 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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