|
| Status |
Public on Dec 21, 2019 |
| Title |
De novo formation in maize from irradiated materials |
| Organism |
Zea mays |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In maize (Zea mays), irradiation can produce many variants with varying centromeric DNA sequences. We found de novo centromeres in F3-152 and F3-600, which has no canonical centromeric repeat sequences. This centromere is derived from a 1520-kb and 188-kb region on the arm of chromosome 10 and 3. Our results suggest that de novo centromere formation is more common than previously thought and can persist on chromosomal fragments without a canonical centromere providing implications for karyotype evolution.
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| |
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| Overall design |
ChIP-seq was carried out with anti-CENH3 antibodies using material from young leaves in B73, F3-152 and F3-600.
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| Contributor(s) |
Han F |
| Citation(s) |
31995554, 32817254 |
| |
| Submission date |
Dec 21, 2018 |
| Last update date |
Oct 13, 2020 |
| Contact name |
Handong Su |
| E-mail(s) |
shandong@mail.hzau.edu.cn
|
| Phone |
+86-18810795796
|
| Organization name |
Huazhong Agricultural University
|
| Street address |
No.1,Shizishan Street ยท Hongshan District
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
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| Platforms (1) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA511397 |
| SRA |
SRP174175 |
