GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE124218 Query DataSets for GSE124218
Status Public on Jun 30, 2020
Title Human Bacille Calmette-Guérin vaccination elicits trained immunity via the hematopoietic progenitor compartment [bone marrow]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Induction of trained immunity by human Bacille-Calmette-Guérin (BCG) vaccination is implicated in the beneficial heterologous effects of the vaccine, but the underlying mechanisms remain elusive. We performed global transcriptome analysis of sorted progenitors from bone marrow before (D0) and 90 days after vaccination (D90). BCG vaccination induced transcriptomic myeloid priming of the hematopoietic stem and progenitor cell (HSPC) compartment marked by the upregulation of myeloid and granulocytic pathways alongside the induction of transcription factors connected to myeloid cell function, namely Hepatocyte Nuclear Factors (HNF). These findings are corroborated by higher granulocyte numbers in BCG-vaccinated infants, HNF1-related SNP variants correlating with immune training and elevated serum levels of the HNF1 target gene SERPINA1. Taken together, we reveal a transcriptomic reprograming of HSPCs and peripheral monocytes as a trait of in vivo BCG-induced trained immunity.
Overall design mRNA profiles of sorted hematopoietic stem and progenitor cells (HSPC, i.e. pooled HSC & MPP) from human bone marrow. Sample collection was performed before and 90 days after vaccination from the same individuals (n=15 for BCG-vaccinated individuals, n=5 for placebo-treated individuals) resulting in a total of 39 transcriptomic profiles (one sample (Donor 9 D0) was identified as outlier and excluded).
Contributor(s) Cirovic B, de Bree CL, Picelli S, Händler K, Klee K, Schultze JL, Netea MG, Schlitzer A
Citation(s) 32544459
Submission date Dec 20, 2018
Last update date May 31, 2021
Contact name Branko Cirovic
Organization name LIMES
Lab Myeloid Cell Biology
Street address Carl-Troll Str 31
City Bonn
ZIP/Postal code 53111
Country Germany
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (39)
GSM3525871 BCG_D20_HSPC_BM_D0
GSM3525872 BCG_D20_HSPC_BM_D90
GSM3525873 Ctrl_D11_HSPC_BM_D0
This SubSeries is part of SuperSeries:
GSE124220 Human Bacille Calmette-Guérin vaccination elicits trained immunity via the hematopoietic progenitor compartment
BioProject PRJNA511004
SRA SRP174103

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124218_BCG_BM_cirovic_counts.txt.gz 5.8 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap