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Series GSE124077 Query DataSets for GSE124077
Status Public on Dec 18, 2019
Title STAT4 activation by leukemia inhibitory factor confers a therapeutic effect on intestinal inflammation [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report that microbiota dysregulation promotes LIF secretion by intestinal epithelial cells (IECs) in a mouse colitis model. LIF greatly activates STAT4 phosphorylation on multiple SPXX elements within the C-terminal transcription regulation domain. STAT4 and STAT3 act reciprocally on both canonical cis-inducible elements (SIEs) and noncanonical “AGG” elements at different loci. In lamina propria lymphocytes (LPLs), STAT4 activation by LIF blocks STAT3-dependent Il-17a/Il-17f promoter activation, whereas in IECs, LIF bypasses the extraordinarily low level of STAT4 to induce YAP gene expression via STAT3 activation. In addition, we found that the administration of LIF is sufficient to restore microbiome homeostasis. Thus, LIF effectively inhibits Th17 accumulation and promotes repair of damaged intestinal epithelium in inflamed colon, and serves as a potential therapy for IBD.
Overall design mRNA profiles in intestinal epithelial cells of wild type (WT) and Stat4-/- mice were generated by deep sequencing, in duplicate, using Illumina-HiSeq2500/4000.
Contributor(s) Zhang YS, Chin YE
Citation(s) 30770344
Submission date Dec 18, 2018
Last update date Mar 19, 2020
Contact name Yanan S Zhang
Phone 86-18817575525
Organization name Soochow University
Department Institutes of Biology and Medical Sciences
Lab Institutes of Biology and Medical Sciences
Street address 199 Renai Road
City Suzhou
State/province Jiangsu
ZIP/Postal code 215123
Country China
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM3520235 IEC-WT-control_1
GSM3520236 IEC-WT-Lif_1
GSM3520237 IEC-S4-KO-control_1
This SubSeries is part of SuperSeries:
GSE124079 STAT4 activation by leukemia inhibitory factor confers a therapeutic effect on intestinal inflammation
BioProject PRJNA510663
SRA SRP173841

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Supplementary file Size Download File type/resource
GSE124077_IECs.GeneExpression.FPKM.xlsx 643.8 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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