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Series GSE123936 Query DataSets for GSE123936
Status Public on Dec 31, 2019
Title Accelerated evolution of oligodendrocytes in human brain
Organisms Macaca mulatta; Pan troglodytes; Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Recent discussions of human brain evolution have largely focused on increased neuron numbers and changes in their connectivity and expression. However, it is increasingly appreciated that oligodendrocytes play important roles in cognitive function and disease. Whether both cell types follow distinctive evolutionary trajectories is not known. We examined the transcriptomes of neurons and oligodendrocytes in the frontal cortex of humans, chimpanzees, and rhesus macaques. We identified human-specific trajectories of gene expression in oligodendrocytes and show that oligodendrocytes have undergone accelerated gene expression evolution in the human lineage. The signature of acceleration is enriched for cell type-specific expression alterations in schizophrenia. These results underscore the importance of oligodendrocytes in human brain evolution.
Overall design We compared the cell-type specific transcriptome profiling of sorted nuclei from humans to chimpanzees, our closest extant relative, using rhesus macaque as an outgroup. We analyzed genome-wide expression levels in adult human Brodmann area 46 (BA46, 27 individuals), and the homologous regions of chimpanzee (11 individuals), and rhesus macaque (15 individuals). Cell-type specific whole transcriptome data was obtained using fluorescence-activated nuclei sorting (FANS) with antibodies to either NeuN or OLIG2 to isolate neurons (NeuN) or oligodendrocytes (OLIG2) and their precursors, respectively.
Contributor(s) Berto S, Usui N, Toriumi K, Douglas C, Konopka G
Citation(s) 31712436, 33795684
Submission date Dec 17, 2018
Last update date Apr 20, 2021
Contact name Genevieve Konopka
Organization name UT Southwestern Medical Center
Department Neuroscience
Street address 5323 Harry Hines Blvd.
City Dallas
State/province TX
ZIP/Postal code 75390-9111
Country USA
Platforms (3)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21120 Illumina NextSeq 500 (Macaca mulatta)
GPL21121 Illumina NextSeq 500 (Pan troglodytes)
Samples (95)
GSM3517099 YN16_043_PanTro_NeuN
GSM3517100 YN16_043_PanTro_Olig2
GSM3517101 YN15_033_PanTro_NeuN
BioProject PRJNA510347
SRA SRP173599

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Supplementary file Size Download File type/resource
GSE123936_HCR_Raw_Count_Exons.txt.gz 1.4 Mb (ftp)(http) TXT
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