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Series GSE123742 Query DataSets for GSE123742
Status Public on Aug 28, 2019
Title RNAseq on Xist-tetOP FL, ΔA, ΔB+C cell lines, with 2 days of differentiation, without or with DOX induction
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Xist RNA has been established as the master regulator of X-chromosome inactivation in female eutherian mammals but its mechanism of action remain unclear. By creating novel Xist mutants at the endogenous locus in mouse embryonic stem cells, we dissect the role of most of the conserved A-F repeats. We find that transcriptional silencing can be uncoupled from Polycomb repressive complex 1 and 2 (PRC1/2) recruitment, which requires repeats B and C. Xist ΔB+C specifically looses interaction with PCGF3/5 subunits of PRC1, while binding of other Xist partners are largely unaffected. However, a slight relaxation of transcriptional silencing in Xist ΔB+C indicates a role for PRC1/2 proteins in early stabilization of gene repression. Distinct structural modules within the Xist RNA are therefore involved in the convergence of independent chromatin modification and gene repression pathways, with Polycomb recruitment only being of moderate relevance in the initiation of silencing.
Overall design RNAseq on Xist-tetOP FL, ΔA, ΔB+C cell line, after 2 days of differentiation, in presence or absence of DOX, in replicates.
Contributor(s) Bousard A, Raposo A, Zylicz JJ, Picard C, Borges Pires V, Qi Y, Syx L, Chang H, Heard E, Teixeira da Rocha S
Citation(s) 31456285
Submission date Dec 12, 2018
Last update date Sep 04, 2019
Contact name Aurélie Bousard
Organization name Institut Curie
Street address 26, rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM3510510 RNAseq_XistTetOP_delBC_D2_noDox_Rep1
GSM3510511 RNAseq_XistTetOP_delBC_D2_noDox_Rep2
GSM3510512 RNAseq_XistTetOP_delBC_D2_Dox_Rep1
This SubSeries is part of SuperSeries:
GSE123743 Xist RNA-dependent Polycomb recruitment through the B and C repeats results in mild relaxation of X-linked transcriptional silencing
BioProject PRJNA509672
SRA SRP173346

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123742_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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