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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 28, 2019 |
| Title |
RNAseq on Xist-tetOP FL, ΔA, ΔB+C cell lines, with 2 days of differentiation, without or with DOX induction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Xist RNA has been established as the master regulator of X-chromosome inactivation in female eutherian mammals but its mechanism of action remain unclear. By creating novel Xist mutants at the endogenous locus in mouse embryonic stem cells, we dissect the role of most of the conserved A-F repeats. We find that transcriptional silencing can be uncoupled from Polycomb repressive complex 1 and 2 (PRC1/2) recruitment, which requires repeats B and C. Xist ΔB+C specifically looses interaction with PCGF3/5 subunits of PRC1, while binding of other Xist partners are largely unaffected. However, a slight relaxation of transcriptional silencing in Xist ΔB+C indicates a role for PRC1/2 proteins in early stabilization of gene repression. Distinct structural modules within the Xist RNA are therefore involved in the convergence of independent chromatin modification and gene repression pathways, with Polycomb recruitment only being of moderate relevance in the initiation of silencing.
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| Overall design |
RNAseq on Xist-tetOP FL, ΔA, ΔB+C cell line, after 2 days of differentiation, in presence or absence of DOX, in replicates.
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| Contributor(s) |
Bousard A, Raposo A, Zylicz JJ, Picard C, Borges Pires V, Qi Y, Syx L, Chang H, Heard E, Teixeira da Rocha S |
| Citation(s) |
31456285 |
| Submission date |
Dec 12, 2018 |
| Last update date |
Sep 04, 2019 |
| Contact name |
Aurélie Bousard |
| E-mail(s) |
aurelie.bousard@curie.fr
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| Organization name |
Institut Curie
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| Street address |
26, rue d'Ulm
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| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE123743 |
Xist RNA-dependent Polycomb recruitment through the B and C repeats results in mild relaxation of X-linked transcriptional silencing |
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| Relations |
| BioProject |
PRJNA509672 |
| SRA |
SRP173346 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE123742_RAW.tar |
1.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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