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| Status |
Public on Dec 01, 2021 |
| Title |
ROS control in human iPS cells reveals early events in spontaneous carcinogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We developed a system to control the intracellular level of reactive oxygen species (ROS) in human iPS cells. By introducing a specific substitution (I69E) into the SDHC protein, a component of the mitochondrial respiratory chain complex, the intracellular ROS level considerably increased. This caused an abnormal development of iPS cells, and these abnormalities were reverted by the overproduction of mitochondria-targeted catalase. When ROS-overproducing iPS cells (ChiPSC12-M cells) were used for subcutaneous inoculation on the backs of nude mice, the mice exhibited tumor formation. The expression of tumor-related FOXC1 transcription factor increased as early as 4 h after the the development of ROS-overproducing iPS cells was initiated. An RNA-seq analysis revealed that 27 transcript clusters among the more than 20,000 transcrips that were examined were highly expressed at 4 h after the initiation of tumor development in ChiPSC12-M cells. Some of these overexpressed transcripts might make networks to activate protooncogenes for spontaneous carcinogenesis.
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| Overall design |
ChiPSC12-M was cultured with Day 0 endoderm differentiation medium and the medium was replaced with Day 1 differentiation medium with or without Tet-Express solution and the cells were cultured for 4 h. Total RNA from each cell was isolated using Isogen (Nippon Gene Co., Ltd, Toyama, Japan) and was subjected to RNA-Sequencing (Cell Innovator, Kyushu Univ., Fukuoka, Japan).
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| Contributor(s) |
Oka S, Hayashi M, Taguchi K, Tsuzuki T, Sekiguchi M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 06, 2018 |
| Last update date |
Dec 01, 2021 |
| Contact name |
Sugako Oka |
| E-mail(s) |
sugako@mbr.nifty.com
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| Organization name |
Fukoka Dental College
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| Street address |
2-15-1, Tamura, Sawara-ku
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| City |
Fukuoka |
| ZIP/Postal code |
814-0193 |
| Country |
Japan |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| GSM3503081 |
Tet-Express-treated ChiPSC12-M, biological rep1 |
| GSM3503082 |
Tet-Express-treated ChiPSC12-M, biological rep2 |
| GSM3503083 |
Tet-Express-treated ChiPSC12-M, biological rep3 |
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| Relations |
| BioProject |
PRJNA508743 |
| SRA |
SRP172734 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE123428_normalized_counts.txt.gz |
752.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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