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Series GSE123125 Query DataSets for GSE123125
Status Public on Aug 28, 2019
Title Chronic Dengue Encephalitis in a Patient with Progressive Dementia
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Dengue virus is the most common arbovirus worldwide and represents a significant public health concern. To date, chronic Dengue infections have not been previously reported. While investigating the etiology of central nervous system (CNS) disease in a patient presenting with progressive dementia, we elucidated a chronic dengue infection within the CNS. Comprehensive viral immune responses in both serum and cerebrospinal fluid (CSF) were profiled by a phage-display assay (VirScan). Enrichment of Dengue viral antibodies were detected in the CSF as compared to the serum. No virus was detected in serum or CSF, but post-mortem analysis confirmed Dengue virus in the brain by quantitative polymerase chain reaction (PCR), immunohistochemistry, RNAscope and sequencing. Dengue virus was detectable by PCR and sequencing from brain biopsy tissue collected 33 months ante-mortem, confirming a chronic infection. Comprehensive antibody profiling assays can aid in the diagnosis of encephalitis of unknown etiologies. Our findings suggest that Dengue virus infections may persist in the CNS and should be considered in patients with progressive dementia in endemic regions or with relevant travel history.
Overall design Microbe profiles for 1 postmortem human cortex clinical sample were generated via deep sequencing using the Illumina NovaSeq6000_S4 system and reagents.
Contributor(s) Johnson TP, Larman HB, Lee MH, Whitehead SS, Kowalak J, Toro C, Kim J, Johnson KR, Reoma LB, Faustin A, Pardo C, Kottapalli S, Howard J, Monaco D, Weisfeld-Adams J, Blackstone C, Galetta S, Snuderl M, Gahl WA, Kister I, Nath A
Citation(s) 31461177
Submission date Nov 29, 2018
Last update date Nov 27, 2019
Contact name Kory R Johnson
Phone 301-402-1956
Organization name NINDS/NIH
Department DIR IT & Bioinformatics
Lab Bioinformatics Section
Street address 10/3B01, 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (1)
GSM3496095 Clinical_Sample
BioProject PRJNA507605
SRA SRP171153

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123125_RAW.tar 330.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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