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Series GSE123052 Query DataSets for GSE123052
Status Public on Jan 06, 2020
Title Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation
Organism Drosophila melanogaster
Experiment type Other
Summary Makorins are evolutionary conserved proteins that contain C3H-type zinc finger modules and a RING E3 ubiquitin ligase domain. In Drosophila maternal Makorin 1 (Mkrn1) has been linked to embryonic patterning but the mechanism remained unsolved. Here, we show that Mkrn1 is essential for axis specification and pole plasm assembly by translational activation of oskar (osk). We demonstrate that Mkrn1 interacts with poly(A) binding protein (pAbp) and binds specifically to osk 3’ UTR in regions overlapping with Bruno1 (Bru1) responsive elements (BREs) that regulate osk translation. We observe increased association of the translational repressor Bru1 with osk mRNA upon depletion of Mkrn1, indicating that both proteins compete for osk binding. Consistently, reducing Bru1 dosage partially rescues viability and Osk protein level in ovaries from Mkrn1 females. We conclude that Mkrn1 controls embryonic patterning and germ cell formation by specifically activating osk translation, most likely by competing with Bru1 to bind to osk 3’ UTR.
Overall design iCLIP analysis of Mkrn1 in S2R+ cells
Contributor(s) Dold A, Hildebrandt A, Busch A, Brüggemann M, Zarnack K, König J, Roignant J
Citation(s) 31978041
Submission date Nov 28, 2018
Last update date Feb 24, 2020
Contact name Annabelle Dold
Organization name Institute of Molecular Biology, IMB
Lab Roignant
Street address Ackermannweg 4
City Mainz
ZIP/Postal code 55128
Country Germany
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (7)
GSM3494419 imb_koenig_2018_06_control
GSM3494420 imb_koenig_2018_06_rep_01
GSM3494421 imb_koenig_2018_06_rep_02
BioProject PRJNA507419
SRA SRP171043

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Supplementary file Size Download File type/resource
GSE123052_RAW.tar 1.9 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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