GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE122886 Query DataSets for GSE122886
Status Public on Nov 26, 2018
Title Transcriptomic analyses of the testicular interstitial cells in murine neonates
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Previous studies have established that fetal Leydig cells (FLCs) and adult Leydig cells (ALCs) show distinct functional characteristics. However, the lineage relationship between FLCs and ALCs has not been clarified yet. Here we reveal that a subset of FLCs dedifferentiate at fetal stages to give rise to ALCs at the pubertal stage. Moreover, the dedifferentiated cells contribute to the peritubular myoid cells and vascular pericyte populations in the neonatal testis, and these non-steroidogenic cells serve as potential ALC stem cells. We generated FLC lineage-specific Nr5a1 (Ad4BP/SF-1) gene disrupted mice and mice lacking the fetal Leydig enhancer (FLE) of the Nr5a1 gene. Phenotypes of these mice support the conclusion that most of the ALCs arise from dedifferentiated FLCs, and that the FLE of Nr5a1 gene is essential for both initial FLC differentiation and pubertal ALC redifferentiation.
Overall design Cells were dispersed from mFLE-Cre;CAG-CAT-EGFP mouse testis at postnatal day 10, and EGFP(weak)/Laminin(negative) population and EGFP(positive)/Laminin(positive) pouplations were collected by cell sorting. Duplicated samples in each population were analyzed by RNA-sequence.
Contributor(s) Shima Y, Miyabayashi K, Ohkawa Y, Sato T, Suyama M
Citation(s) 30518625
Submission date Nov 25, 2018
Last update date Feb 25, 2019
Contact name Yuichi Shima
Phone +81 942 31 7541
Organization name Kurume University School of Medicine
Department Department of Anatomy
Lab Division of Microscopic and Developmental Anatomy
Street address Asahimachi 67
City Kurume
State/province Fukuoka
ZIP/Postal code 830-0011
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (4)
GSM3487659 P10-EGFP-plus-Lam-mius-1
GSM3487660 P10-EGFP-plus-Lam-mius-2
GSM3487661 P10-EGFP-plus-Lam-plus-1
BioProject PRJNA506821
SRA SRP170612

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122886_TableS4.txt.gz 1017.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap