GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE122588 Query DataSets for GSE122588
Status Public on Mar 06, 2019
Title iPSC-Derived Brain Endothelium Exhibits Stable, Long-Term Barrier Function in Perfused Hydrogel Scaffolds
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We describe the construction a 3D blood-brain barrier model comprised of iPSC-derived brain endothelium cultured in channels within gelatin hydrogels. The iPSC-derived brain endothelium displays key features of the blood-brain barrier phenotype, including tight junction formation, efflux transporter activity, low paracellular permeability, and suppressed levels of transcytosis compared to non-blood-brain barrier endothelial controls. Collectively, this work provides the foundation for a fully human, biomimetic neurovascular model to study BBB in health and disease.
Overall design iPSC-derived brain endothelium was cultured in 3D channels in gelatin hydrogels and analyzed for key signatures of blood-brain barrier phenotype. RNA-seq was used to assess changes in global gene expression signatures between endothelium cultured for one day under static conditions and fourteen days under perfused conditions.
Contributor(s) Neal EH, Faley SL, Bellan LM, Lippmann ES
Citation(s) 30773484
Submission date Nov 15, 2018
Last update date Mar 06, 2019
Contact name Ethan Lippmann
Organization name Vanderbilt University
Department Chemical and Biomolecular Engineering
Lab Lippmann Lab
Street address 2400 Highland Ave, Room 303
City Nashville
State/province TN
ZIP/Postal code 37212
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM3475305 static_BMEC
GSM3475306 perfused_BMEC
BioProject PRJNA505675
SRA SRP168818

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122588_full_3D_table.csv.gz 7.4 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap