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Series GSE122587 Query DataSets for GSE122587
Status Public on Oct 01, 2022
Title Insights into the mechanism underlying the therapeutic effects of raspberry ketone on human colorectal carcinoma cells revealed by RNA sequencing and bioinformatics analysis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Colorectal cancer (CRC) is the most commonly diagnosed cancer of the digestive system. Bioactive substances from plants are of particular research interest, due to their chemopreventive or chemotherapeutic properties against tumors. Raspberry ketone (RK), one of the major aromatic compounds in red raspberries, is widely used as a flavoring agent in foodstuffs, and for nutritional and medicinal purposes. In the present study, we found that RK inhibited the growth of DLD-1 CRC cells and identified differentially expressed genes (DEGs) by RNA sequencing in DLD-1 cells treated with RK. Transcriptome analyses indicated significant changes in the mRNA levels of 901 genes according to P - values < 0.01 (609 up- and 292 down-regulated) after treatment of DLD-1 cells with 400 μg/mL RK for 72 h. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that various cellular functions were involved in RK treatment. Interaction network analysis of GO terms and KEGG pathways revealed details of their interactions and predicted the key RK target pathways. These results provide evidence applicable to the prevention and therapy of CRC.
Overall design Identified differentially expressed genes (DEGs) by RNA sequencing in DLD-1 cells treated with RK
Contributor(s) Zhang HP, Liu M
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Submission date Nov 15, 2018
Last update date Oct 03, 2022
Contact name Haopeng Zhang
Phone +86451-82576685
Organization name The Fourth Affiliated Hospital of Harbin Medical University
Department Department of General Surgery & Bio-Bank of General Surgery
Street address No. 37 Yiyuan Street
City Harbin
State/province Heilongjiang
ZIP/Postal code 150001
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (5)
GSM3475300 DLD1-control-1
GSM3475301 DLD1-control-2
GSM3475302 DLD1-treatment-1
BioProject PRJNA505676
SRA SRP168765

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Supplementary file Size Download File type/resource
GSE122587_RAW.tar 22.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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