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Series GSE122457 Query DataSets for GSE122457
Status Public on Apr 03, 2020
Title Endogenous siRNAs Promote Proteostasis and Longevity in Germline-less C. elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary How lifespan and the rate of aging are set is a key problem in biology. Small RNAs are conserved molecules that impact diverse biological processes through the control of gene expression. However, in contrast to miRNAs, the role of endo-siRNAs in aging remains unexplored. Here, by combining deep sequencing and genomic and genetic approaches in C. elegans, we reveal an unprecedented role for endo-siRNA molecules in the maintenance of proteostasis and lifespan extension in germline-less animals. Furthermore, we identify an endo-siRNA-regulated tyrosine phosphatase, which limits the longevity of germline-less animals by restricting the activity of the heat shock transcription factor HSF-1. Altogether, our findings point to endo-siRNAs as a link between germline removal and the HSF-1 proteostasis and longevity-promoting somatic pathway. This establishes a role for endo siRNAs in the aging process and identifies downstream genes and physiological processes that are regulated by the endo siRNAs to affect longevity.
Overall design Two-condition experiment, Wild type (N2) C. elegans vs. dcr-1(mg375) C. elegans, glp-1(e2144) C. elegans vs. dcr-1(mg375) glp-1(e2144) C. elegans. Data from four biological replicates were used for each strain.
Contributor(s) Cohen Berkman M, Ben-Hemo S,  Fishman A, Waldman Ben-Asher H,  Lamm AT, Henis-Korenblit S
Citation(s) 32213289
Submission date Nov 13, 2018
Last update date Apr 06, 2020
Contact name Hiba Waldman Ben-Asher
Organization name Bar-Ilan University
Department Life-Sciences
Street address Ramat-Gan
City Ramat-Gan
ZIP/Postal code 52900
Country Israel
Platforms (1)
GPL25800 Agilent-020186 C.elegans v2 (GeneName version)
Samples (8)
GSM3466755 glp-1(e2144) Caenorhabditis elegans vs. dcr-1(mg375) Caenorhabditis elegans [WIS_252018610640_1_1]
GSM3466756 Wild type (N2) Caenorhabditis elegans vs. dcr-1(mg375) glp-1(e2144) Caenorhabditis elegans [WIS_252018610640_1_2]
GSM3466757 glp-1(e2144) Caenorhabditis elegans vs. dcr-1(mg375) Caenorhabditis elegans [WIS_252018610640_1_3]
BioProject PRJNA505282

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122457_RAW.tar 109.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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