|
| Status |
Public on Apr 03, 2020 |
| Title |
Endogenous siRNAs Promote Proteostasis and Longevity in Germline-less C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
How lifespan and the rate of aging are set is a key problem in biology. Small RNAs are conserved molecules that impact diverse biological processes through the control of gene expression. However, in contrast to miRNAs, the role of endo-siRNAs in aging remains unexplored. Here, by combining deep sequencing and genomic and genetic approaches in C. elegans, we reveal an unprecedented role for endo-siRNA molecules in the maintenance of proteostasis and lifespan extension in germline-less animals. Furthermore, we identify an endo-siRNA-regulated tyrosine phosphatase, which limits the longevity of germline-less animals by restricting the activity of the heat shock transcription factor HSF-1. Altogether, our findings point to endo-siRNAs as a link between germline removal and the HSF-1 proteostasis and longevity-promoting somatic pathway. This establishes a role for endo siRNAs in the aging process and identifies downstream genes and physiological processes that are regulated by the endo siRNAs to affect longevity.
|
| |
|
| Overall design |
Two-condition experiment, Wild type (N2) C. elegans vs. dcr-1(mg375) C. elegans, glp-1(e2144) C. elegans vs. dcr-1(mg375) glp-1(e2144) C. elegans. Data from four biological replicates were used for each strain.
|
| |
|
| Contributor(s) |
Cohen Berkman M, Ben-Hemo S, Fishman A, Waldman Ben-Asher H, Lamm AT, Henis-Korenblit S |
| Citation(s) |
32213289 |
| |
| Submission date |
Nov 13, 2018 |
| Last update date |
Apr 06, 2020 |
| Contact name |
Hiba Waldman Ben-Asher |
| Organization name |
Bar-Ilan University
|
| Department |
Life-Sciences
|
| Street address |
Ramat-Gan
|
| City |
Ramat-Gan |
| ZIP/Postal code |
52900 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL25800 |
Agilent-020186 C.elegans v2 (GeneName version) |
|
| Samples (8)
|
| GSM3466755 |
glp-1(e2144) Caenorhabditis elegans vs. dcr-1(mg375) Caenorhabditis elegans [WIS_252018610640_1_1] |
| GSM3466756 |
Wild type (N2) Caenorhabditis elegans vs. dcr-1(mg375) glp-1(e2144) Caenorhabditis elegans [WIS_252018610640_1_2] |
| GSM3466757 |
glp-1(e2144) Caenorhabditis elegans vs. dcr-1(mg375) Caenorhabditis elegans [WIS_252018610640_1_3] |
| GSM3466758 |
Wild type (N2) Caenorhabditis elegans vs. dcr-1(mg375) glp-1(e2144) Caenorhabditis elegans [WIS_252018610640_1_4] |
| GSM3466759 |
Wild type (N2) Caenorhabditis elegans vs. dcr-1(mg375) Caenorhabditis elegans [WIS_252018610642_1_1] |
| GSM3466760 |
Wild type (N2) Caenorhabditis elegans vs. dcr-1(mg375) Caenorhabditis elegans [WIS_252018610642_1_2] |
| GSM3466761 |
glp-1(e2144) Caenorhabditis elegans vs. dcr-1(mg375) glp-1(e2144) Caenorhabditis elegans [WIS_252018610642_1_3] |
| GSM3466762 |
glp-1(e2144) Caenorhabditis elegans vs. dcr-1(mg375) glp-1(e2144) Caenorhabditis elegans [WIS_252018610642_1_4] |
|
| Relations |
| BioProject |
PRJNA505282 |
Less...
More...