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Series GSE122176 Query DataSets for GSE122176
Status Public on Aug 26, 2019
Title Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Astrocytic morphogenesis and maturation are critical steps in CNS development. The time window of astrocyte morphological development is well defined, but the molecular underpinnings are still unclear. BDNF is a critical growth factor involved in the development of the CNS, including synapse refinement. Here we demonstrate the BDNF receptor at Ntrk2 is enriched in astrocytes relative to all CNS cell populations. RNA sequencing indicates Ntrk2 falls in the top 0.001% of all gene transcripts expressed in juvenile astrocytes, almost exclusively due to truncated TrkB.T1. Astrocyte complexity is increased in the presence of BNDF in vitro, which is dependent upon the presence of TrkB.T1. Furthermore, deletion of TrkB.T1 in vivo revealed astrocytes with significantly reduced volume and branching complexities. Indicating a role for functional astrocyte maturation via BDNF/TrkB.T1 signaling, TrkB.T1 KO astrocytes do not support normal excitatory synaptogenesis. Together, these data suggest a significant role for BDNF/TrkB.T1 signaling in astrocyte morphogenesis and indicate this signaling may contribute to astrocyte regulation of neuronal synapse development.
Overall design Wild-type (WT) males were bred with heterozygous Mecp2tm1.1Jae/+ (Jaenisch) female mice. Astrocytes were acutely isolated from whole cortex as previously described (Holt and Olsen, 2016)) from WT males ranging from postnatal days 25-31. An n of 5 biological replicates were used. For RNA-Sequencing, 2 technical replicates were run per biological replicate.
Contributor(s) Pacheco NL, Olsen M
Citation(s) 31433295
Submission date Nov 05, 2018
Last update date Aug 28, 2019
Contact name Michelle L. Olsen
Phone (540) 231-7394
Organization name Virginia Polytechnic and State University
Department School of Neuroscience
Lab LS1 RM 213(540)
Street address 970 Washington Street SW
City Blacksburg
State/province VA
ZIP/Postal code 24060
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (5)
GSM3457269 P28 WT Astrocyte rep 1
GSM3457270 P28 WT Astrocyte rep 2
GSM3457271 P28 WT Astrocyte rep 3
BioProject PRJNA503873
SRA SRP167875

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Supplementary file Size Download File type/resource
GSE122176_RAW.tar 6.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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