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Series GSE121972 Query DataSets for GSE121972
Status Public on Nov 01, 2020
Title Whole-body-vibration training positively affects muscle transcriptome in tumour bearing cachectic mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Background The prevailing view is that a combination of nutritional, pharmacological and exercise therapy is most optimal to reduce the development and progression of cancer cachexia. However, in many cancer patients, physical activity is hampered by frailty and fatigue. The present study aimed to investigate whole-body vibration training (WBV) as potential alternative to attenuate loss of weight, muscle mass and function in tumour bearing mice. Methods Twenty-four male CD2F1-mice (21.5±0.2g) were stratified into four groups (control=[C], control+WBV=[C+V], tumour-bearing=[T] and tumour-bearing+WBV=[T+V]), and injected (day 1) with C26 cells or vehicle. From day 1, whole-body-vibration (WBV groups) was performed for 19 days (15min, 45Hz, 1.0g acceleration). General outcome measures included body mass and composition, and daily activity. In addition, blood analysis and assessments of muscle histology and function and whole genome gene expression in m. soleus (SOL) and m. extensor digitorum longus (EDL) were performed. Two-way ANOVA with factors tumour, training and interaction was used for statistical analysis. Results Body weight, lean and fat mass and EDL mass were all lower in tumour bearing mice compared to controls. No effects of vibration training were found on systemic cachexia related outcomes. However, WBV increased EDL mass in the control treatment,. SOL mass did not differ, whereas SOL function was affected by both tumour and WBV. Interestingly, WBV reduced the tumour-related effects on muscle gene expression in EDL, SOL and heart. Conclusions These data suggest that WBV had only minor effects on cachexia related outcomes in the present experimental set-up, while muscle transcriptome was positively affected. This merits follow-up studies applying for example longer treatment periods or incorporating WBV in a multiple-target intervention.
Overall design Microarray analysis was performed on skeletal muscle biopsies (m. soleus, m. extensor digitorum longus and heart) from mice with or without cachexia and/or whole body vibration training.
Contributor(s) Plas RL, van Dijk M, Dwarkasing JT, van Gemerden F, Swarts HJ, van Schothorst EM, Witkamp RF, van Norren K
Citation(s) 34732809
Submission date Oct 30, 2018
Last update date Nov 10, 2021
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (58)
GSM3452052 EDL, Tumour bearing, replicate 1
GSM3452053 EDL, Tumour bearing + WBV, replicate 4
GSM3452054 EDL, Control + WBV, replicate 4
BioProject PRJNA501868

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Supplementary file Size Download File type/resource
GSE121972_RAW.tar 249.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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