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Status |
Public on Dec 27, 2018 |
Title |
Comparative gene expression profiling of the three main types of mouse dendritic cells isolated ex vivo from the spleen versus generated in vitro in bone marrow FLT3-L cultures |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array Third-party reanalysis
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Summary |
The goal of this experiment was to use global gene expression profiling to compare the transcriptomic profiles between the three major types of dendritic cells (DCs) directly isolated ex vivo from the spleen versus derived in vitro from bone marrow FLT3-L cultures. The three DC types analyzed in each condition were type 1 classical DCs (cDC1s), type 2 cDCs (cDC2s) and plasmacytoid DCs (pDCs). The gene expression profiles of the samples of the in vitro derived DCs used here were not reported before. We have previously reported the gene expression profiles of the samples used here for the DCs directly isolated ex vivo from the spleen. They correspond to a reanalysis of the following records: cDC1: GSM1828801 and GSM1828802, cDC2: GSM3178486 and GSM3178487, pDC: GSM2882708 and GSM2882709.
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Overall design |
DCs were generated in vitro from mouse bone marrow FLT3-L cultures and sorted by flow cytometry to over 98% purity, as live, singlet, CD11c+ cells that were SiglecH(+) for eq-pDCs, SIRPĪ±(-/low)CD24(high) for eq-cDC1s and SIRPĪ±(high)CD24(-/low) for eq-cDC2s.
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Contributor(s) |
Sanchez C, Vu Manh T, Dalod M |
Citation(s) |
30564233 |
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Submission date |
Oct 26, 2018 |
Last update date |
Mar 04, 2019 |
Contact name |
Thien-Phong Vu Manh |
Organization name |
CIML
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Street address |
163 avenue de Luminy
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City |
marseille |
ZIP/Postal code |
13288 |
Country |
France |
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Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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Samples (8)
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Relations |
Reanalysis of |
GSM1828801 |
Reanalysis of |
GSM1828802 |
Reanalysis of |
GSM3178486 |
Reanalysis of |
GSM3178487 |
Reanalysis of |
GSM2882708 |
Reanalysis of |
GSM2882709 |
BioProject |
PRJNA498672 |