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Series GSE121859 Query DataSets for GSE121859
Status Public on Dec 27, 2018
Title Comparative gene expression profiling of the three main types of mouse dendritic cells isolated ex vivo from the spleen versus generated in vitro in bone marrow FLT3-L cultures
Organism Mus musculus
Experiment type Expression profiling by array
Third-party reanalysis
Summary The goal of this experiment was to use global gene expression profiling to compare the transcriptomic profiles between the three major types of dendritic cells (DCs) directly isolated ex vivo from the spleen versus derived in vitro from bone marrow FLT3-L cultures. The three DC types analyzed in each condition were type 1 classical DCs (cDC1s), type 2 cDCs (cDC2s) and plasmacytoid DCs (pDCs). The gene expression profiles of the samples of the in vitro derived DCs used here were not reported before. We have previously reported the gene expression profiles of the samples used here for the DCs directly isolated ex vivo from the spleen. They correspond to a reanalysis of the following records: cDC1: GSM1828801 and GSM1828802, cDC2: GSM3178486 and GSM3178487, pDC: GSM2882708 and GSM2882709.
 
Overall design DCs were generated in vitro from mouse bone marrow FLT3-L cultures and sorted by flow cytometry to over 98% purity, as live, singlet, CD11c+ cells that were SiglecH(+) for eq-pDCs, SIRPĪ±(-/low)CD24(high) for eq-cDC1s and SIRPĪ±(high)CD24(-/low) for eq-cDC2s.
 
Contributor(s) Sanchez C, Vu Manh T, Dalod M
Citation(s) 30564233
Submission date Oct 26, 2018
Last update date Mar 04, 2019
Contact name Thien-Phong Vu Manh
Organization name CIML
Street address 163 avenue de Luminy
City marseille
ZIP/Postal code 13288
Country France
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (8)
GSM3448069 A13026_CD11b_UNS_1
GSM3448070 A13026_CD11b_UNS_2
GSM3448071 A13026_CD11b_UNS_3
Relations
Reanalysis of GSM1828801
Reanalysis of GSM1828802
Reanalysis of GSM3178486
Reanalysis of GSM3178487
Reanalysis of GSM2882708
Reanalysis of GSM2882709
BioProject PRJNA498672

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE121859_RAW.tar 31.2 Mb (http)(custom) TAR (of CEL)
GSE121859_processed_data_allsamples.txt.gz 4.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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