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Series GSE121830 Query DataSets for GSE121830
Status Public on Oct 04, 2019
Title RNA-sequencing with micro-dissected boundary organoid into anterior, posterior, and boundary regions
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report the continuous patterning and dynamic morphogenesis of hepatic, biliary and pancreatic structures, invaginating from a three-dimensional culture of human pluripotent stem cell (PSC). The boundary interactions between anterior and posterior gut spheroids differentiated from human PSCs enables autonomous emergence of hepato-biliary-pancreatic (HBP) organ domains specified at the foregut-midgut boundary organoids in the absence of extrinsic factor supply. The regional identity of the boundary region of anterior-posteior spheroids was analyzed by RNA-sequencing with micro-dissected boundary organoid into anterior, posterior, and boundary regions. Each region expressed the corresponding gut lineage markers. The early HBP specification markers including HHEX1 and PDX1 at the boundary were highly upregulated compared with the other two regions, indicating that the anterior-posterior boundary interactions autonomously generate HHEX and PDX1 positive cells in the absence of exogenous inductive factors. To delineate the HBP progenitor self-inductive mechanism, we evaluated the boundary specific expression profiles of known inductive signaling pathways that includes FGF, BMP, Hedgehog, NOTCH and retinoic acid (RA) signals, and found that the signal downstream of RA were activated prominently at the boundary region but not in the anterior or posterior regions.
Overall design Examination of mRNA expression profile in micro-dissected 3 different region from boundary organoid.
Contributor(s) Takebe T, Koike H, Saiki N, Iwasawa K
Citation(s) 31554966
Submission date Oct 26, 2018
Last update date Oct 04, 2019
Contact name Norikazu Saiki
Phone +81-466-32-1904
Organization name Tokyo Medical and Dental University
Department Institute of Research
Street address 1-5-45 Yushima, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8510
Country Japan
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (15)
GSM3447561 D11 A
GSM3447562 D11 B
GSM3447563 D11 P
BioProject PRJNA498598
SRA SRP166932

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Supplementary file Size Download File type/resource
GSE121830_RAW.tar 7.8 Mb (http)(custom) TAR (of TXT, XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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