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Series GSE121760 Query DataSets for GSE121760
Status Public on Jul 31, 2019
Title Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Meiosis is the specialized cell division during which parental genomes recombine to create genotypically unique gametes. Despite its importance, mammalian meiosis cannot be studied in vitro, greatly limiting mechanistic studies. In vivo, meiocytes progress asynchronously through meiosis and therefore the study of specific stages of meiosis is a challenge. Here, we describe a simple method for isolating pure sub-populations of meiocytes that allows for detailed study of meiotic sub-stages. Interrogating the H3K4me3 landscape revealed dynamic chromatin transitions between sub-stages of meiotic prophase I, both at sites of genetic recombination and at gene promoters. We also leveraged this method to perform the first comprehensive, genome-wide survey of histone marks in meiotic prophase, revealing a heretofore unappreciated complexity of the epigenetic landscape at meiotic recombination hotspots. Ultimately, this study presents a simple, scalable framework for interrogating the complexities of mammalian meiosis.
Overall design We devised a method to isolate specific populations of meiotic nuclei from whole testis of mice. We subsequently interrogated the dynamic histone modification landscape using ChIP-Seq.
Contributor(s) Lam KG, Brick K, Cheng G, Pratto F, Camerini-Otero RD
Citation(s) 31444359
Submission date Oct 24, 2018
Last update date Sep 03, 2019
Contact name Kevin Brick
Organization name NIDDK
Department GBB
Street address 5/205 Memorial Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (51)
GSM3734408 H3K4me3 ChIP-Seq Leptotene nuclei (Expt R1)
GSM3734409 H3K9Ac ChIP-Seq Leptotene nuclei (Expt R1)
GSM3734410 input ChIP-Seq Leptotene nuclei (Expt R1)
BioProject PRJNA498571
SRA SRP166913

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Supplementary file Size Download File type/resource
GSE121760_RAW.tar 28.3 Gb (http)(custom) TAR (of BED, BIGWIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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