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Status |
Public on Oct 01, 2019 |
Title |
Role of iron in the regulation of the epithelial-to-mesenchymal transition (ChIP-seq) |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The biological process termed Epithelial-to-Mesenchymal Transition (EMT) plays a central role in cancer cell invasion, metastasis, self-renewal and resistance to therapy. Here, we characterize using chromatin immunoprecipitation and Next Generation Sequencing the genome-wide H3K9me2 distribution occurring during EMT induced by epidermal growth factor in breast cancer MDA-MB-468 cells.
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Overall design |
Analysis of 9 samples: (i)Input DNA to normalize samples; (ii) H3K9me2 Immunoprecipitated in untreated cells and (iii) H3K9me2 immunoprecipitated in EGF-treated cells. Experiment was performed in 3 independent biological replicates.
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Contributor(s) |
Sindikubwabo F, Servant N, Baulande S, Raphael R |
Citation(s) |
32747755 |
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Submission date |
Oct 23, 2018 |
Last update date |
Jul 09, 2021 |
Contact name |
Nicolas Servant |
E-mail(s) |
Nicolas.Servant@curie.fr
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Organization name |
Institut Curie
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Street address |
26 rue d'ulm
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City |
Paris Cedex 05 |
ZIP/Postal code |
75248 |
Country |
France |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (22)
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This SubSeries is part of SuperSeries: |
GSE121664 |
Role of iron in the regulation of the epithelial-to-mesenchymal transition |
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Relations |
BioProject |
PRJNA498059 |
SRA |
SRP166453 |