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Series GSE121605 Query DataSets for GSE121605
Status Public on Apr 29, 2019
Title Requirements for multivalent Yb body assembly in transposon silencing in Drosophila
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Female sterile (1) Yb (Yb) is a primary component of Yb bodies, perinuclear foci known as the site of PIWI-interacting RNA (piRNA) biogenesis in Drosophila ovarian somatic cells. Yb consists of Helicase C-terminal (Hel-C), RNA helicase and extended Tudor (eTud) domains. We previously showed that the RNA helicase domain is necessary for Yb−RNA interaction, Yb body formation and piRNA biogenesis. Here, we investigated the functions of the two other domains and found that Hel-C and eTud are necessary for Yb to self-associate and to interact with RNAs and other Yb body components, respectively. Both domains were essential for Yb body formation and transposon silencing. Without eTud, piRNA production was completely impaired. Loss of Hel-C severely reduced the levels of transposon-targeting piRNAs, although genic piRNAs unrelated to transposon silencing were still produced. Similar phenotypes were observed when mutations in flamenco, the primary source of transposon-targeting piRNAs, led to the expression of attenuated RNAs. Yb bodies are liquid-like multivalent condensates whose assembly depends on Yb self-association and widespread Yb−flamenco RNA binding.
Overall design Four piRNA-seq libraries: piRNA libraries were prepared from RNA associated with Flag-Piwi immunoprecipitates (with size selection at 23-30 nt).
Contributor(s) Hirakata S, Siomi MC
Citation(s) 31267711
Submission date Oct 22, 2018
Last update date Jul 21, 2019
Contact name Shigeki Hirakata
Organization name The University of Tokyo
Department Department of Biological Sciences
Lab Siomi Lab
Street address 2-11-16 Yayoi, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0032
Country Japan
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (4)
GSM3439984 WT_Yb_rep_1
GSM3439985 WT_Yb_rep_2
GSM3439986 ΔHel-C_rep1
BioProject PRJNA497868
SRA SRP166327

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Supplementary file Size Download File type/resource
GSE121605_processed_data.txt.gz 90.1 Kb (ftp)(http) TXT
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