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Status |
Public on Aug 31, 2021 |
Title |
NF-κB1 (p50) impairs macrophage driven inflammatory response of the host against Helicobacter pylori infection |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
In the present study, we observed spontaneous gastritis in 10-12 week old NF-κB1—/— mice with increased level of pro-inflammatory cytokines in gastric tissues. The NF-κB1—/— bone marrow derived macrophages showed increased level of cytokines which further exacerbated with H. pylori infection. To map the contribution of macrophages in the spontaneous gastritis, we performed comparative transcriptome analysis of NF-κB1—/— mice gastric macrophage and H. pylori treated macrophages. The transcriptome analysis showed an upregulation of innate immune response genes in NF-κB1—/— gastric macrophages, but not in H. pylori activated murine macrophages. Network analysis identified STAT1/TLR7/TLR13 as a key regulator and inhibition of STAT1 activation attenuated exacerbated innate immune response. NF-κB1—/— mice after infection with H. pylori showed increased histological gastritis and reduced level of H. pylori colonization. Our study indicates that H. pylori-mediated up-regulation of NF-κB1 mitigates host immune response and promotes chronic colonization of the pathogen.
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Overall design |
Comparative RNA profiles of H. pylori ex-vivo activated mice gastric macrophages and NF-κB1 knock-out/wild type mice gastric macrophages
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Contributor(s) |
Gupta S, Ponnusamy K, Gupta J, Tiwari R, Pandey A, Kumari R, Pandey J, Chaturvedi M, Kumar Ray A, Kirti Mishra S, Tandon R, Habib T, Kumar Dhar P, Chaturvedi R |
Citation missing |
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Submission date |
Oct 22, 2018 |
Last update date |
Aug 31, 2021 |
Contact name |
Tanwir Habib |
E-mail(s) |
thabib@sidra.org
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Organization name |
Sidra Medicine
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Street address |
Al Luqta Street
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City |
Doha |
ZIP/Postal code |
26999 |
Country |
Qatar |
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Platforms (1) |
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Samples (8)
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Relations |
BioProject |
PRJNA497835 |
SRA |
SRP166298 |
Supplementary file |
Size |
Download |
File type/resource |
GSE121583_genes.fpkm.tracking.xls.gz |
5.6 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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