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Series GSE121583 Query DataSets for GSE121583
Status Public on Aug 31, 2021
Title NF-κB1 (p50) impairs macrophage driven inflammatory response of the host against Helicobacter pylori infection
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In the present study, we observed spontaneous gastritis in 10-12 week old NF-κB1—/— mice with increased level of pro-inflammatory cytokines in gastric tissues. The NF-κB1—/— bone marrow derived macrophages showed increased level of cytokines which further exacerbated with H. pylori infection. To map the contribution of macrophages in the spontaneous gastritis, we performed comparative transcriptome analysis of NF-κB1—/— mice gastric macrophage and H. pylori treated macrophages. The transcriptome analysis showed an upregulation of innate immune response genes in NF-κB1—/— gastric macrophages, but not in H. pylori activated murine macrophages. Network analysis identified STAT1/TLR7/TLR13 as a key regulator and inhibition of STAT1 activation attenuated exacerbated innate immune response. NF-κB1—/— mice after infection with H. pylori showed increased histological gastritis and reduced level of H. pylori colonization. Our study indicates that H. pylori-mediated up-regulation of NF-κB1 mitigates host immune response and promotes chronic colonization of the pathogen.
Overall design Comparative RNA profiles of H. pylori ex-vivo activated mice gastric macrophages and NF-κB1 knock-out/wild type mice gastric macrophages
Contributor(s) Gupta S, Ponnusamy K, Gupta J, Tiwari R, Pandey A, Kumari R, Pandey J, Chaturvedi M, Kumar Ray A, Kirti Mishra S, Tandon R, Habib T, Kumar Dhar P, Chaturvedi R
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Submission date Oct 22, 2018
Last update date Aug 31, 2021
Contact name Tanwir Habib
Organization name Sidra Medicine
Street address Al Luqta Street
City Doha
ZIP/Postal code 26999
Country Qatar
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (8)
GSM3439677 Wt_1
GSM3439678 Wt_2
GSM3439679 NF-κB1(p50)_1
BioProject PRJNA497835
SRA SRP166298

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Supplementary file Size Download File type/resource
GSE121583_genes.fpkm.tracking.xls.gz 5.6 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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