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Series GSE121386 Query DataSets for GSE121386
Status Public on Nov 07, 2018
Title Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary IL-10 is a prototypical anti-inflammatory cytokine, which is fundamental to the maintenance of immune homeostasis, especially in the intestine. There is an assumption that cells producing IL-10 have an immunoregulatory function. However, here we report that IL-10-producing CD4+ T cells are phenotypically and functionally heterogeneous. By combining single cell transcriptome and functional analyses, we identified a subpopulation of IL-10-producing Foxp3Neg CD4+ T cells that displays regulatory activity unlike other IL-10-producing CD4+ T cells, which are unexpectedly pro-inflammatory. The combinatorial expression of co-inhibitory receptors is sufficient to discriminate IL-10-producing CD4+ T cells with regulatory function from others and to identify them across different tissues and disease models in mice and humans. These regulatory IL-10-producing Foxp3Neg CD4+ T cells have a unique transcriptional program, which goes beyond the regulation of IL-10 expression. Finally, we found that patients with Inflammatory Bowel Disease (IBD), demonstrate a deficiency in this specific regulatory T-cell subpopulation.
 
Overall design We isolated T-cells from the spleen and small intestine of mice treated with anti-CD3. We sorted the IL-10+ CD4+ T-cells and sequenced them on a single cell level using the SMART-seq2 protocol.
 
Contributor(s) Brockmann L, Soukou S, Steglich B, Czarnewski P, Can E, Villablanca EJ, Gagliani N, Huber S
Citation(s) 30575716
Submission date Oct 17, 2018
Last update date Jan 27, 2019
Contact name Babett Steglich
Organization name Universitätsklinikum Hamburg-Eppendorf
Street address Martinistrasse 52
City Hamburg
ZIP/Postal code 20246
Country Germany
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (764)
GSM3433684 IL-10+ CD4+ T-cells small intestine p2_A13
GSM3433685 IL-10+ CD4+ T-cells small intestine p2_A14
GSM3433686 IL-10+ CD4+ T-cells small intestine p2_A15
This SubSeries is part of SuperSeries:
GSE121393 Molecular and functional heterogeneity of IL-10-producing CD4+ T cells
Relations
BioProject PRJNA497250
SRA SRP165988

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Supplementary file Size Download File type/resource
GSE121386_SI.counts.txt.gz 1.4 Mb (ftp)(http) TXT
GSE121386_spleen.counts.txt.gz 4.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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