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| Status |
Public on Jan 01, 2020 |
| Title |
Gene expression profiling of wiltype and Ctr1 knockout MEFs with depleted cellular Cu levels |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The transition metal Cu is an essential micronutrient in biological systems. The majority of Cu is taken up into the cell through the Cu transporter CTR1 and thus, deletion of Ctr1 results in Cu deficiency. Here we performed transcriptional profiling of isogenic mouse embryonic fibroblast harboring conditional alleles for Ctr1 floxed out or intact through in vitro administration of doxycycline. The goal was to elucidate gene expression changes upon knockout of Ctr1.
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| Overall design |
Gene expression by RNAseq of isogenic mouse embryonic fibroblasts (MEFs) either wildtype or null for Ctr1 in triplicate. In addition, MEFs null for Ctr1 were independently treated with 500mM of the Cu chelator BCS for 48 hours in triplicate. Total of 9 samples were analyzed.
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| Contributor(s) |
Brady DC, Tsang T |
| Citation missing |
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| |
| Submission date |
Oct 16, 2018 |
| Last update date |
Jan 01, 2020 |
| Contact name |
Donita Colette Brady |
| E-mail(s) |
bradyd@pennmedicine.upenn.edu
|
| Phone |
2155739705
|
| Organization name |
University of Pennsylvania
|
| Department |
Cancer Biology
|
| Lab |
Brady
|
| Street address |
421 Curie Blvd. BRBII/III Room 612
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA497055 |
| SRA |
SRP165861 |
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