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| Status |
Public on Nov 07, 2018 |
| Title |
Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse 10x] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
IL-10 is a prototypical anti-inflammatory cytokine, which is fundamental to the maintenance of immune homeostasis, especially in the intestine. There is an assumption that cells producing IL-10 have an immunoregulatory function. However, here we report that IL-10-producing CD4+ T cells are phenotypically and functionally heterogeneous. By combining single cell transcriptome and functional analyses, we identified a subpopulation of IL-10-producing Foxp3Neg CD4+ T cells that displays regulatory activity unlike other IL-10-producing CD4+ T cells, which are unexpectedly pro-inflammatory. The combinatorial expression of co-inhibitory receptors is sufficient to discriminate IL-10-producing CD4+ T cells with regulatory function from others and to identify them across different tissues and disease models in mice and humans. These regulatory IL-10-producing Foxp3Neg CD4+ T cells have a unique transcriptional program, which goes beyond the regulation of IL-10 expression. Finally, we found that patients with Inflammatory Bowel Disease (IBD), demonstrate a deficiency in this specific regulatory T-cell subpopulation.
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| Overall design |
We isolated T-cells from the spleen and small intestine of mice treated with anti-CD3. We sorted the IL-10+ CD4+ T-cells and loaded them onto the 10X Genomics Chromium platform for single-cell capture. cDNA libraries were constructed and subsequently sequenced on the Illumina platform.
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| Contributor(s) |
Brockmann L, Soukou S, Steglich B, Czarnewski P, Can E, Villablanca EJ, Gagliani N, Huber S |
| Citation(s) |
30575716 |
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| Submission date |
Oct 15, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Babett Steglich |
| Organization name |
Universitätsklinikum Hamburg-Eppendorf
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| Street address |
Martinistrasse 52
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| City |
Hamburg |
| ZIP/Postal code |
20246 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE121393 |
Molecular and functional heterogeneity of IL-10-producing CD4+ T cells |
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| Relations |
| BioProject |
PRJNA496523 |
| SRA |
SRP165797 |
