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Series GSE121229 Query DataSets for GSE121229
Status Public on Apr 19, 2019
Title BRCA1 Mutations Attenuate Super-Enhancer Function and Chromatin Looping in Haploinsufficient Human Breast Epithelial Cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary BRCA1 functions in multiple biological processes, including double-strand break repair, replication stress suppression, transcriptional regulation, and chromatin reorganization. While non-malignant cells carrying cancer-predisposing BRCA1 mutations exhibit increased genomic instability, it remains unclear whether BRCA1 haploinsufficiency affects transcription and chromatin dynamics. Here we show that primary mammary epithelial cells from women with BRCA1 mutations (BRCA1mut/+) display significant loss of H3K27ac-associated super-enhancers.
Overall design Primary human mammary epithelial cells (HMECs) were isolated from fresh cancer-free breast tissues of BRCA1 mutation carriers (BRCA1mut/+, n = 3) and non-carriers (BRCA1+/+, n = 3), who underwent prophylactic mastectomy and reduction mammoplasty, respectively. H3K27ac chromatin immunoprecipitation with deep-sequencing (ChIP-seq) was performed.
Contributor(s) Zhang X, Wang Y, Chiang H, Li R
Citation(s) 30995943
Submission date Oct 15, 2018
Last update date Apr 21, 2019
Contact name Rong Li
Organization name University of Texas Health Science Center at San Antonio
Department Molecular Medicine
Street address 7703 Floyd Curl Drive
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM3428171 BSC112: H3K27ac_NC_1
GSM3428172 BSC122L: H3K27ac_NC_2
GSM3428173 BSC121R: H3K27ac_NC_3
BioProject PRJNA496412
SRA SRP165726

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Supplementary file Size Download File type/resource
GSE121229_RAW.tar 20.0 Kb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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