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Series GSE120761 Query DataSets for GSE120761
Status Public on Apr 01, 2019
Title Cyp2b-null male mice are susceptible to high-fat diet-induced obesity due to changes in PUFA metabolism and response to hepatic lipids as measured by RNAseq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the role of CYP2B in lipid metabolism, a Cyp2b triple knockout mouse lacking Cyp2b9, Cyp2b10, and Cyp2b13 was developed using CRISPER/Cas9. Wildtype (WT) and Cyp2b-null mice were fed a normal diet (ND) or a 60% high-fat diet (HFD) for 10 weeks. RNA was extracted from the livers of male and female mice from all treatment groups and used for RNA seqencing. RNAseq data demonstrated that hepatic gene expression in ND-fed Cyp2b-null male mice is similar to HFD-fed WT mice, indicating that Cyp2b-null male mice are reacting as if they are receiving a HFD even if they are not. Gene ontology and KEGG pathways show perturbations in lipid metabolism pathways, including PUFA metabolism, fatty acid elongation, and glycerophospholipid metabolism.
Overall design Use RNA-sequencing to investigate the role of Cyp2b in hight-fat diet-induced obesity on a transcriptomic level, by comparing the livers of WT and Cyp2b-null mice fed a HFD for 10 weeks using Illumina technology.
Contributor(s) Baldwin WS, Heintz MM, Noorai RE
Citation(s) 31202118
NIH grant(s)
Grant ID Grant title Affiliation Name
R15 ES017321 Does the inhibition of key xenobiotic-metabolizing CYPs cause obesity? CLEMSON UNIVERSITY William S Baldwin
P20 GM109094 COBRE:Eukaryotic Pathogens Innovation Center (EPIC) CLEMSON UNIVERSITY LESLY A TEMESVARI
Submission date Oct 02, 2018
Last update date Jul 01, 2019
Contact name William S Baldwin
Phone 915-491-7366
Organization name Clemson University
Department Biological Sciences
Lab Dr. William Baldwin
Street address 230 Parkway Dr
City Clemson
State/province South Carolina
ZIP/Postal code 29634
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (24)
GSM3414895 WT_ND_1_male
GSM3414896 WT_ND_2_male
GSM3414897 WT_ND_3_male
BioProject PRJNA494405
SRA SRP163144

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120761_Raw_counts_Cyp2bnull_HFD.txt.gz 617.6 Kb (ftp)(http) TXT
GSE120761_Rawcounts_Female_Cyp2bnull_HFD_study.txt.gz 485.7 Kb (ftp)(http) TXT
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Processed data are available on Series record

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