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Series GSE120710 Query DataSets for GSE120710
Status Public on Oct 23, 2018
Title A three-dimensional organoid model recapitulates tumorigenic aspects and drug responses of advanced human retinoblastoma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Third-party reanalysis
Summary Persistent or recurrent retinoblastoma (RB) is associated with the presence of vitreous or/and subretinal seeds in advanced RB and represents a major cause of therapeutic failure. This necessitates the development of novel therapies and thus requires a model of advanced RB for testing candidate therapeutics. To this aim, we established and characterized a three-dimensional, self-organizing organoid model derived from chemotherapy-naïve tumors. The responses of organoids to drugs were determined and compared to relate organoid model to advanced RB, in terms of drug sensitivities. We found that organoids had histological features resembling retinal tumors and seeds and retained DNA copy-number alterations as well as gene expression profile and protein expression of the parental tissue. Cone signal circuitry (M/L+ cells) and glial tumor microenvironment (GFAP+ cells) were primarily present in organoids. Topotecan alone or the combined drug regimen of topotecan and melphalan effectively targeted proliferative tumor cones (RXRg+ Ki67+) in organoids after 24-h drug exposure, blocking mitotic entry. In contrast, methotrexate showed the least efficacy against tumor cells. The drug responses of organoids were consistent with those of tumor cells in advanced disease. Patient-derived organoids enable the creation of a faithful model to use in examining novel therapeutics for RB.
Overall design Samples from human retinoblastoma organoids [2 samples from passages 1 (6-week culture) and 3 (13-week culture)] and retinoblastoma tissue [the tumor tissue used for culture (1 sample)] were used to generate RNA-seq libraries. Gene expression profiles were generated by sequencing using NovaSeq6000.
Published gene expression profiles of fetal retina (2 samples, days 132 and 136 from Hoshino et al., 2017; Dev Cell; 43(6):763-779.e4) and retinoblastoma (1 sample from Aldiri et al., 2017; Neuron; 94(3):550-568.e10) were included for analysis. The three reanalyzed samples are included in supplementary file kaewkhaw.tumor.organoids.668.Hsap38.Ens82.gene.CPM.tsv linked at the foot of this record.
Contributor(s) Kaewkhaw R
Citation(s) 30353124
Submission date Oct 01, 2018
Last update date Nov 05, 2018
Contact name Rossukon Kaewkhaw
Phone +66 (662) 201 1000
Organization name Mahidol University
Street address 270 Rama VI Road Ratchathewi
City Bangkok
ZIP/Postal code 10400
Country Thailand
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (3)
GSM3408882 RB668 Tumor tissue
GSM3408883 RB668 organoids_P1
GSM3408884 RB668 organoids_P3
Reanalysis of GSM2319605
Reanalysis of GSM2808450
Reanalysis of GSM2808451
BioProject PRJNA494224
SRA SRP163050

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Supplementary file Size Download File type/resource
GSE120710_kaewkhaw.tumor.organoids.668.Hsap38.Ens82.gene.CPM.tsv.gz 3.9 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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