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Series GSE120667 Query DataSets for GSE120667
Status Public on Nov 30, 2018
Title Acc1 determines memory potential of individual CD4+ T cells by regulating de novo fatty acid biosynthesis [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Immunological memory is central to adaptive immunity and protection from disease. Changing metabolic demands as antigen-specific T cells transition from effector to memory have been well documented, but the cell-specific pathways and molecules that govern this transition are poorly defined. ACC1 is a rate-limiting enzym in fatty acid biosynthesis. We show that genetic deletion of ACC1 enhanced the formation of CD4+ T cell memory, and that inhibition of ACC1 function enhanced memory T cell formation during parasite infection in mice. ACC1-deficient effector Th cells had similar metabolic signatures to wild-type memory Th cells, and Acaca expression was inversely correlated with a memory gene signature in individual cells. Single cell analysis allowed us to identify a memory precursor-enriched population (CCR7hiCD137lo) present during early differentiation of effector CD4+ T cells. Thus, fatty acid metabolism directs cell fate determination during the generation of memory CD4+ T cells.
 
Overall design Phenotypic characterization of CCR7hiCD137lo and CCR7loCD137hi CD4+ T cell populations.
 
Contributor(s) Endo Y, Onodera A, Obata-Ninomiya K, Koyama-Nasu R, Asou HK, Ito T, Yamamoto T, Kanno T, Nakajima T, Ishiwata K, Kanuka H, Tumes DJ, Nakayama T
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Submission date Oct 01, 2018
Last update date Dec 02, 2018
Contact name Yusuke ENDO
E-mail(s) san3tamariayuyu@chiba-u.jp
Phone 81432262186
Organization name Chiba University
Department Immunology
Lab Immunology
Street address 1-8-1 Inohana, Chuo-ku
City Chiba-city
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (4)
GSM3408029 CCR7hiCD137lo-1_ RNA
GSM3408030 CCR7loCD137hi-1_ RNA
GSM3408031 CCR7hiCD137lo-2_ RNA
This SubSeries is part of SuperSeries:
GSE122863 Acc1 determines memory potential of individual CD4+ T cells by regulating de novo fatty acid biosynthesis
Relations
BioProject PRJNA494052
SRA SRP162980

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120667_RAW.tar 2.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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