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Series GSE120462 Query DataSets for GSE120462
Status Public on Nov 28, 2018
Title Single cell transcriptional profiles of control and Ikzf2-transfected mouse cochlear cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In order to determine the transcriptional effect of Ikzf2 overexpression in mammalian auditory hair cells, mouse cochleae were transfected with either a control GFP or a Ikzf2 virus between postnatal day 1 and 3 (P1-3) and then harvested for single cell gene expression profiling at postnatal day 8 (P8) on the 10X Genomics Single Cell 3' v2 platform.
Overall design Dataset is composed of two separate single cell RNA-Seq samples captured on the 10X Genomics Chromium platform with the Single Cell 3' Solution v2 chemistry. Viral GFP- (control) or Ikzf2-transfected cochleae were harvested and single cell suspensions prepared from transgenic mice expressing tdTomato in hair cells. Hair cells expressing tdTomato were enriched by flow sorting and then captured on the Chromium system in parellel. Library preparation and sequencing was performed as defined by the 10X Genomics Single Cell 3' Solution v2 protocol.
Contributor(s) Mattern MS, Kelly MC
Citation(s) 30464345
Submission date Sep 25, 2018
Last update date Mar 25, 2019
Contact name Michael C Kelly
Organization name NCI
Department Center for Cancer Research
Lab Single Cell Analysis Facility
Street address 41 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20894
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM3401092 GFP
GSM3401093 Ikzf2
BioProject PRJNA493093
SRA SRP162647

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120462_RAW.tar 10.0 Mb (http)(custom) TAR (of H5)
GSE120462_mm10plusCustom_genes.gtf.gz 25.4 Mb (ftp)(http) GTF
GSE120462_mm10plusCustom_genome.fa.gz 769.1 Mb (ftp)(http) FA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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