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Series GSE120444 Query DataSets for GSE120444
Status Public on Sep 26, 2018
Title Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry [bulk]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Bulk RNA Sequencing of Healthy Bone Marrow Mononuclear Cells
Overall design Using standard operating procedures, mononuclear cells from bone marrow aspirates were isolated using Ficoll density gradient separation and cryopreserved in 90% FBS/ 10% DMSO for storage in liquid nitrogen. RNA was harvested from thawed cell vials of BMMCs using AllPrep kits (QIAGEN). Libraries were prepared using TruSeq Stranded Total RNA Sample Preparation Kit (Illumina) with 1ug of RNA input. Sequencing was performed by paired-end 75 nt on Illumina HiSeq 3000.
Contributor(s) Oetjen KA, Hourigan CS
Citation(s) 30518681
Submission date Sep 25, 2018
Last update date Dec 23, 2018
Contact name Christopher Hourigan
Phone 3014510257
Organization name National Heart Lung and Blood Institute, NIH
Department Hematology Branch
Lab Myeloid Malignancies
Street address Room 10CRC 5-5130, 10 Center Drive
City Bethesda
State/province Maryland
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (8)
GSM3400913 A
GSM3400914 B
GSM3400915 C
This SubSeries is part of SuperSeries:
GSE120446 Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry
BioProject PRJNA493081
SRA SRP162552

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120444_RAW.tar 42.3 Mb (http)(custom) TAR (of H5, JSON, TSV)
GSE120444_RNASeqCounts.txt.gz 1.1 Mb (ftp)(http) TXT
GSE120444_xCelloutput.txt.gz 977 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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