|
| Status |
Public on Sep 26, 2018 |
| Title |
Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry [bulk] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bulk RNA Sequencing of Healthy Bone Marrow Mononuclear Cells
|
| |
|
| Overall design |
Using standard operating procedures, mononuclear cells from bone marrow aspirates were isolated using Ficoll density gradient separation and cryopreserved in 90% FBS/ 10% DMSO for storage in liquid nitrogen. RNA was harvested from thawed cell vials of BMMCs using AllPrep kits (QIAGEN). Libraries were prepared using TruSeq Stranded Total RNA Sample Preparation Kit (Illumina) with 1ug of RNA input. Sequencing was performed by paired-end 75 nt on Illumina HiSeq 3000.
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| |
|
| Contributor(s) |
Oetjen KA, Hourigan CS |
| Citation(s) |
30518681 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| ZIA HL006163 |
Detection, prevention and treatment of acute myeloid leukemia (AML) relapse. |
National Heart, Lung, and Blood Institute |
Christopher Simon Hourigan |
|
| |
| Submission date |
Sep 25, 2018 |
| Last update date |
Dec 23, 2018 |
| Contact name |
Laura Dillon |
| E-mail(s) |
laura.dillon2@nih.gov
|
| Organization name |
National Heart Lung and Blood Institute, NIH
|
| Department |
Hematology Branch
|
| Lab |
Myeloid Malignancies
|
| Street address |
Room 10CRC 6-5142, 10 Center Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE120446 |
Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry |
|
| Relations |
| BioProject |
PRJNA493081 |
| SRA |
SRP162552 |
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