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| Status |
Public on Apr 30, 2019 |
| Title |
Next Generation Sequencing analysis atg5 gene expression in zebrafish model |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: study of function of ATG5 protein on zebrafish larvae development
Methods: Using morpholino oligos for atg5 gene knock down and overexpression, injected into one- to four-cell-stage embryos,extracted total RNA, then sending to RNA sequencing.
Results: we compared to Wildtype, 5MO and atg5 mRNA groups, we analyza autophagy related gene mRNA expression, there are obvious different.
Conclusion: our study showed the function of autophagy related gene 5 on zebrafish larvae, it has an important significant for understanding the affection of autophagy on embryo development
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| |
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| Overall design |
Using morpholino oligos for atg5 gene knock down and overexpression, injected into one- to four-cell-stage embryos,extracted total RNA, then sending to RNA sequencing.
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| |
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| Contributor(s) |
Hu Z |
| Citation(s) |
30992325 |
| |
| Submission date |
Sep 19, 2018 |
| Last update date |
May 02, 2019 |
| Contact name |
zhanying hu |
| E-mail(s) |
zhanying.hu@bblumberg.org
|
| Organization name |
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College
|
| Street address |
1# tiantanxili, dongcheng district
|
| City |
beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
| |
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| Platforms (1) |
| GPL21741 |
Illumina HiSeq 4000 (Danio rerio) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA491832 |
| SRA |
SRP162109 |
