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Series GSE120021 Query DataSets for GSE120021
Status Public on Sep 19, 2018
Title Gene expression profiling of xenografts generated by lung metastasis screening models
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Understanding the roles of ncRNAs in tumorigenesis and metastasis would establish avenues for the identification of diagnostic and therapeutic targets. We observed that lncRNA SNHG10 and its derived SCARNA13 were concomitantly upregulated in hepatocellular carcinoma (HCC) pulmonary metastases and associated with poor prognosis. Mechanistically, SCARNA13, modulated by SNHG10, facilitated the cell cycle and epithelial-mesenchymal transition (EMT) of HCC cells by promoting SOX9. SNHG10 served as a sponge for miR-150-5p and interacted with RPL4 mRNA to increase the expression and activity of c-Myb. Reciprocally, upregulated and hyperactivated c-Myb enhanced SNHG10 and SCARNA13 expression through regulating SNHG10 promoter activity, forming a positive feedback loop and continuously stimulating SCARNA13 expression. Our findings characterized a complex epigenetic cause of HCC with clinical implications.
Overall design To identify ncRNAs involved in HCC metastasis, we grafted Hep3B and SK-Hep1 cells into nude mice and performed a screen of lung metastasis based on orthotopic implanted models in vivo. RNA-seq was conducted to compare the expression profiles between xenografts and parental HCC cells.
Contributor(s) Lan T, Yan X, Xu L, Liao H
Citation(s) 31101763
Submission date Sep 17, 2018
Last update date May 24, 2019
Contact name Tian Lan
Organization name West China Hospital
Department Department of Liver Surgery & Liver Transplantation
Lab Laboratory of Liver Surgery
Street address No. 37 of Guoxue Lane
City Chengdu
State/province Sichuan
ZIP/Postal code 610041
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM3391773 Hep3B rep 1
GSM3391774 Hep3B rep 2
GSM3391775 SK-Hep1 rep 1
This SubSeries is part of SuperSeries:
GSE120096 Gene expression profiling of xenografts generated by lung metastasis screening models and HCCLM3 cells
BioProject PRJNA491643
SRA SRP161993

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120021_RAW.tar 16.5 Mb (http)(custom) TAR (of TXT)
GSE120021_gene_count.xlsx.gz 6.0 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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