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| Status |
Public on Aug 06, 2020 |
| Title |
Targets of Hnf4alpha during conversion from mouse embryonic fibroblasts (MEFs) to hepatocyte-like (iHep) cells. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used ChIP-seq to explore the targets of Hnf4alpha during conversion event from MEFs to iHep cells.
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| Overall design |
Using an anti-Hnf4alpha antibody we performed ChIP-Seq for iHep_4a1 cells (Foxa1/Hnf4alpha), iHep_4a2 cells (Foxa2/Hnf4alpha), iHep_4a3 cells (Foxa3/Hnf4alpha).
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| Contributor(s) |
Suzuki A, Ohkawa Y, Nagasaki M, Horisawa K, Ueno K |
| Citation(s) |
32755593 |
| Submission date |
Sep 12, 2018 |
| Last update date |
Aug 10, 2020 |
| Contact name |
Atsushi Suzuki |
| Organization name |
Medical Institute of Bioregulation, Kyushu University
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| Department |
Division of Organogenesis and Regeneration
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| Street address |
3-1-1 Maidashi, Higashi-ku
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| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
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| Platforms (2) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA490545 |
| SRA |
SRP161614 |
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