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Series GSE119880 Query DataSets for GSE119880
Status Public on Aug 06, 2020
Title Targets of Hnf4alpha during conversion from mouse embryonic fibroblasts (MEFs) to hepatocyte-like (iHep) cells.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used ChIP-seq to explore the targets of Hnf4alpha during conversion event from MEFs to iHep cells.
Overall design Using an anti-Hnf4alpha antibody we performed ChIP-Seq for iHep_4a1 cells (Foxa1/Hnf4alpha), iHep_4a2 cells (Foxa2/Hnf4alpha), iHep_4a3 cells (Foxa3/Hnf4alpha).
Contributor(s) Suzuki A, Ohkawa Y, Nagasaki M, Horisawa K, Ueno K
Citation(s) 32755593
Submission date Sep 12, 2018
Last update date Aug 10, 2020
Contact name Atsushi Suzuki
Organization name Medical Institute of Bioregulation, Kyushu University
Department Division of Organogenesis and Regeneration
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM3386256 iHep_4a1_2_Hnf4a_R225
GSM3386257 iHep_4a1_2_input_R225
GSM3386258 iHep_4a2_2_Hnf4a_R242
BioProject PRJNA490545
SRA SRP161614

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Supplementary file Size Download File type/resource
GSE119880_RAW.tar 420.3 Mb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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