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Series GSE119670 Query DataSets for GSE119670
Status Public on Sep 08, 2018
Title The expression of MHC class II molecules on murine breast tumors delays T cell exhaustion, expands the T cell repertoire and slows tumor growth
Organism Mus musculus
Experiment type Other
Summary Transfection with the human class II transcriptional activator (hCIITA) promotes MHCII expression on TS/A breast cancer cells. Using a murine breast tumor line, we showed that MHCII-expressing tumors grew more slowly than controls and recruited more functional CD4+ and CD8+ T cells. Additionally, MHCII-expressing tumors contained more TCR clonotypes expanded to a larger degree than control tumors. Functional CD8+ T cells in tumors depended on CD4+ T cells. However, both CD4+ and CD8+ T cells eventually became exhausted, even in MHCII-expressing tumors. Treatment with anti-CTLA4, but not anti-PD-1 or anti-TIM-3, promoted complete eradication of MHCII-expressing tumors. These results suggest tumor cell expression of MHCII facilitates the local activation of CD4+ T cells, indirectly helps the activation and expansion of CD8+ T cells and, in combination with the appropriate checkpoint inhibitor, promotes tumor regression.
Overall design To test whether local MHCII expression promoted T cell clonal expansion or accumulation, we harvested 12 control and 12 MHCII-expressing whole tumor samples and sequenced the CDR3 region of the TCRbeta chain to identify unique T cell clones.
Contributor(s) McCaw TR, Li M, Starenki D, Cooper SJ, Liu M, Meza-Perez S, Arend RC, Buchsbaum DJ, Forero A, Randall TD
Citation(s) 30334128, 30485278
Submission date Sep 07, 2018
Last update date Jan 27, 2019
Contact name Dmytro Starenki
Phone 2563275265
Organization name HudsonAlpha Institute for Biotechnology
Street address 601 Genome Way
City Huntsville
State/province Alabama
ZIP/Postal code 35806
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (2)
GSM3380667 3990-DS-0005
GSM3380668 3990-DS-0006
BioProject PRJNA489881
SRA SRP160388

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Supplementary file Size Download File type/resource
GSE119670_RAW.tar 6.5 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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