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Series GSE119412 Query DataSets for GSE119412
Status Public on Jul 05, 2019
Title Gene expression and alternative splicing profile of MFAP1- and THOC1-depleted human cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary THO/TREX is a conserved complex with a role in messenger ribonucleoprotein biogenesis that links gene expression and genome instability. Here we show that human THO interacts with MFAP1, a spliceosome-associated factor. Interestingly, MFAP1 depletion impairs cell proliferation and genome integrity, increasing γH2AX foci and DNA breaks. This phenotype is not dependent either on transcription or RNA-DNA hybrids. Mutations in the yeast orthologous gene SPP381, also confer a similar transcription-independent genome instability supporting a conserved role. MFAP1 depletion has a wide effect on splicing and gene expression in human cells, determined by transcriptome analyses, that affects a number of DNA damage response (DDR) genes, which supports an indirect role of MFAP1 on genome integrity. Our work defines a novel functional interaction between THO and RNA processing and argues that splicing factors may contribute to genome integrity indirectly by regulating the expression of DDR genes rather than by a direct role.
HeLa cells were depleted of MFAP1 or THOC1 by siRNA during 72 h and their global gene expression and alternative splicing profiles were analyzed.
Data were used to describe genes that were up- or down-regulated or showed alternative splicing changes after MFAP1 or THOC1 depletion.
Overall design We analyzed RNA from control cells (siC), MFAP1-depleted cells (siMFAP1) and THOC1-depleted cells (siTHOC1). For each condition, microarray analysis was conducted in triplicate from three different biological replicates (Total = 9 samples). We compared siMFAP1 vs siC, siTHOC1 vs siC and siMFAP1 vs siTHOC1.
Contributor(s) Salas-Armenteros I, Pérez M, Andújar E, Luna R, Aguilera A
Citation(s) 31390568
Submission date Sep 04, 2018
Last update date Aug 26, 2019
Contact name Andrés Aguilera
Organization name CABIMER, Universidad de Sevilla
Department Genome BIology
Lab Genome Instability & Cancer
Street address Av. Américo Vespucio 24
City Seville
ZIP/Postal code 41092
Country Spain
Platforms (2)
GPL17585 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version]
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (18)
GSM3374431 HeLa cells transfected with control siC for 72 h, biological rep1
GSM3374432 HeLa cells transfected with control siC for 72 h, biological rep2
GSM3374433 HeLa cells transfected with control siC for 72 h, biological rep3
BioProject PRJNA489195

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119412_RAW.tar 475.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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