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Series GSE119308 Query DataSets for GSE119308
Status Public on Aug 31, 2021
Title Aberrant chromatin landscape upon loss of the H3.3 chaperone Daxx leads to Pu.1-mediated neutrophilia and inflammation [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The lymphoid and myeloid lineages are in equilibrium during steady-state hematopoiesis. Tilting hematopoiesis towards innate immunity has been linked to inflammation and may predispose to myeloproliferative disease. Although epigenetic players have been implicated in the control of hematopoiesis and pathogenesis of blood neoplasms, the role of chromatin-based mechanisms in regulating the lymphoid/myeloid balance remains only partially understood. Here, we show that loss of the H3.3 chaperone Daxx causes myeloid skewing, neutrophilia and pyoderma gangrenosum-like lesions, a human skin disease of unknown etiology. Daxx deficiency leads to chromatin opening at intergenic regions, including TERRA target sites, deregulation of repeat elements and activation of anti-viral defense pathways. Finally, the other main H3.3 chaperone, Hira, is dispensable for normal hematopoiesis but supports expansion of Daxx-deficient neutrophils. These results define a role for Daxx in proper selection of lymphoid versus myeloid lineages, linked to control of repeat elements and anti-inflammatory responses.
Overall design Examination of gene expression profiles and chromatin accessibility in hematopoietic stem/progenitor cells comparing wild-type and Daxx knock-out cells.
Contributor(s) Hofmann J, Russ J, Spear S, Guzzi N, Chandrasekar V, Izotova N, Maida S, Händler K, Adams P, Marafioti T, Enver T, Wenzel J, Beyer M, Bellodi C, Schultze J, Capasso M, Nimmo R, Salomoni P
Citation(s) 34876685
Submission date Aug 31, 2018
Last update date Jan 11, 2022
Contact name Paolo Salomoni
Organization name DZNE e.V.
Street address Sigmund-Freud-Str. 27
City Bonn
ZIP/Postal code 53127
Country Germany
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (59)
GSM3369667 Daxx +/+ KLS 1 [3421]
GSM3369668 Daxx +/+ KLS 2 [3422]
GSM3369669 Daxx F/F KLS 1 [3423]
This SubSeries is part of SuperSeries:
GSE119309 Aberrant chromatin landscape upon loss of the H3.3 chaperone Daxx leads to Pu.1-mediated neutrophilia and inflammation
BioProject PRJNA488720
SRA SRP159237

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119308_180829_GMP_NormalizedCounts_AllGenes.txt.gz 590.8 Kb (ftp)(http) TXT
GSE119308_180829_GMP_NormalizedCounts_TEtranscripts.txt.gz 33.9 Kb (ftp)(http) TXT
GSE119308_180829_KLS_NormalizedCounts_AllGenes.txt.gz 597.9 Kb (ftp)(http) TXT
GSE119308_180829_KLS_NormalizedCounts_TEtranscripts.txt.gz 34.1 Kb (ftp)(http) TXT
GSE119308_210215_GMP_24wpi_NormalizedCounts_Gene.txt.gz 983.1 Kb (ftp)(http) TXT
GSE119308_210215_GMP_3wpi_NormalizedCounts_Gene.txt.gz 1.1 Mb (ftp)(http) TXT
GSE119308_210215_GMP_Pu1FFvsWT_3wpi_NormalizedCounts_Gene.txt.gz 727.6 Kb (ftp)(http) TXT
GSE119308_210215_KLS_24wpi_NormalizedCounts_Gene.txt.gz 989.9 Kb (ftp)(http) TXT
GSE119308_210215_KLS_3wpi_NormalizedCounts_Gene.txt.gz 1.2 Mb (ftp)(http) TXT
GSE119308_210215_KLS_Pu1FFvsWT_3wpi_NormalizedCounts_Gene.txt.gz 813.9 Kb (ftp)(http) TXT
GSE119308_210215_LTHSC_3dpi_Rosa_NormalizedCounts_Genes.txt.gz 934.5 Kb (ftp)(http) TXT
GSE119308_210215_LTHSC_3dpi_Rosa_NormalizedCounts_TE.txt.gz 44.7 Kb (ftp)(http) TXT
GSE119308_210215_MPP_3wpi_NormalizedCounts_Gene.txt.gz 1.2 Mb (ftp)(http) TXT
GSE119308_GMP_AllGenotypes.cntTable.txt.gz 13.6 Kb (ftp)(http) TXT
GSE119308_KLS_DaxxFFPu1FFvsDaxxWt.cntTable.txt.gz 12.8 Kb (ftp)(http) TXT
GSE119308_KLS_DaxxFFvsDaxxWt.cntTable.txt.gz 14.3 Kb (ftp)(http) TXT
GSE119308_Mx1_TC.cntTable.txt.gz 24.9 Kb (ftp)(http) TXT
GSE119308_Pu1singleKO_wt1.cntTable.txt.gz 18.5 Kb (ftp)(http) TXT
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