GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE119157 Query DataSets for GSE119157
Status Public on Aug 29, 2018
Title Effect of norepinephrine and focal adhesion kinase (FAK) knockdown in prostate cancer
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Chronic stress is associated with hormonal alterations that are known to promote cancer progression. The stress hormone norepinephrine promotes migration and metastasis of prostate cancer cells. Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase which is phosphorylated during chronic stress or norepinephrine treatment. Here, we investigated how norepinephrine modulates the gene expression in Myc-CaP prostate cancer cell line. We also focused on the effect of FAK knockdown in norepinephrine-induced changes of the gene expression profile.
Overall design Myc-CaP cells stably transfected with shRNAs targeting FAK or negative control were treated with 10μM norepinephrine or vehicle for 24 hours. The effect on gene expression was assessed by RNA-Seq.
Contributor(s) Cheng Y, Yang Y
Citation(s) 30504424
Submission date Aug 28, 2018
Last update date Mar 08, 2019
Contact name Yan Cheng
Organization name University of Arkansas for Medical Sciences
Department Internal Medicine
Lab Fenghuang Zhan Lab
Street address 4301 W. Markham St.
City Little Rock
State/province Arkansas
ZIP/Postal code 72205
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM3359727 shFAKctr
GSM3359728 shFAKNE
GSM3359729 shNCctr
BioProject PRJNA488279
SRA SRP159027

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119157_gene_count.txt.gz 2.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap