GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE118563 Query DataSets for GSE118563
Status Public on Nov 23, 2018
Title CNOT6L Couples the Selective Degradation of Maternal Transcriptsto Meiotic Cell Cycle Progression in Mouse Oocyte [polysome RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goals of this study are to study the function of Cnot6l during oocyte maturation .
Methods: Comparing the polysome-bounded transcripts at GV, MI and MII stage in WT and Cnot6l-/- oocytes by RNA sequencing.
Results: Using an optimized data analysis workflow, we mapped about 15 million sequence reads per sample to the mouse genome (build mm9) and identified 23236 transcripts with TopHat workflow.
Conclusions: CNOT6L stimulated degradation of maternal transcriptsto oocyte meiotic maturation.
Overall design 2 oocytes at GV ,MI and MII stages of WT and Cnot6l-/- mice are performed polysome-bounded transcripts by RNA sequencing.
Contributor(s) Sha Q, Yu J, Guo J, Dai X, Jun-Chao J, Zhang Y, Yu C, Ji S, Jiang Y, Zhang S, Shen L, Ou X, Fan H
Citation(s) 30478191
Submission date Aug 15, 2018
Last update date Mar 01, 2019
Contact name Li Shen
Phone 86-0571-88981751
Organization name Life Sciences Institute, Zhejiang University
Lab Shenlab
Street address 866 Yuhangtang Road
City Hangzhou
State/province Zhejiang Province
ZIP/Postal code 310058
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (12)
GSM3333277 WT_GV_1 [polysome RNA-seq]
GSM3333278 WT_GV_2 [polysome RNA-seq]
GSM3333279 WT_MI_1 [polysome RNA-seq]
This SubSeries is part of SuperSeries:
GSE118564 CNOT6L Couples the Selective Degradation of Maternal Transcriptsto Meiotic Cell Cycle Progression in Mouse Oocyte
BioProject PRJNA486095
SRA SRP157969

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE118563_All_FPKM_table.txt.gz 980.8 Kb (ftp)(http) TXT
GSE118563_RAW.tar 6.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap