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Series GSE118563 Query DataSets for GSE118563
Status Public on Nov 23, 2018
Title CNOT6L Couples the Selective Degradation of Maternal Transcriptsto Meiotic Cell Cycle Progression in Mouse Oocyte [polysome RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goals of this study are to study the function of Cnot6l during oocyte maturation .
Methods: Comparing the polysome-bounded transcripts at GV, MI and MII stage in WT and Cnot6l-/- oocytes by RNA sequencing.
Results: Using an optimized data analysis workflow, we mapped about 15 million sequence reads per sample to the mouse genome (build mm9) and identified 23236 transcripts with TopHat workflow.
Conclusions: CNOT6L stimulated degradation of maternal transcriptsto oocyte meiotic maturation.
 
Overall design 2 oocytes at GV ,MI and MII stages of WT and Cnot6l-/- mice are performed polysome-bounded transcripts by RNA sequencing.
 
Contributor(s) Sha Q, Yu J, Guo J, Dai X, Jun-Chao J, Zhang Y, Yu C, Ji S, Jiang Y, Zhang S, Shen L, Ou X, Fan H
Citation(s) 30478191
Submission date Aug 15, 2018
Last update date Mar 01, 2019
Contact name Li Shen
E-mail(s) shenlab@zju.edu.cn
Phone 86-0571-88981751
Organization name Life Sciences Institute, Zhejiang University
Lab Shenlab
Street address 866 Yuhangtang Road
City Hangzhou
State/province Zhejiang Province
ZIP/Postal code 310058
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (12)
GSM3333277 WT_GV_1 [polysome RNA-seq]
GSM3333278 WT_GV_2 [polysome RNA-seq]
GSM3333279 WT_MI_1 [polysome RNA-seq]
This SubSeries is part of SuperSeries:
GSE118564 CNOT6L Couples the Selective Degradation of Maternal Transcriptsto Meiotic Cell Cycle Progression in Mouse Oocyte
Relations
BioProject PRJNA486095
SRA SRP157969

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE118563_All_FPKM_table.txt.gz 980.8 Kb (ftp)(http) TXT
GSE118563_RAW.tar 6.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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