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Series GSE118534 Query DataSets for GSE118534
Status Public on Oct 11, 2019
Title FACT mediates cohesin function on chromatin
Organisms Saccharomyces cerevisiae; Nakaseomyces glabratus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Cohesin is a key regulator of genome architecture with roles in sister chromatid cohesion and the organisation of higher-order structures during interphase and mitosis. The recruitment and mobility of cohesin complexes on DNA are restricted by nucleosomes. Here we show that cohesin role in chromosome organization requires the histone chaperone FACT. Depletion of FACT in metaphase cells affects cohesin stability on chromatin reducing its accumulation at pericentric regions and binding on chromosome arms. Using Hi-C, we show that cohesin-dependent TAD (Topological Associated Domains)-like structures in G1 and metaphase chromosomes are disrupted in the absence of FACT. Surprisingly, sister chromatid cohesion is intact in FACT-depleted cells, although chromosome segregation failure is observed. Our results uncover a role for FACT in genome organisation by facilitating cohesin dependent compartmentalization of chromosomes into loop domains.
 
Overall design Study of cohesin distribution and chromosome architecture in the presence and absence of the FACT complex subunit Spt16
 
Contributor(s) Garcia-Luis J, Aragon L
Citation(s) 31582854, 36196991
Submission date Aug 14, 2018
Last update date Oct 28, 2022
Contact name Jonay Garcia-Luis
E-mail(s) jonaygl@gmail.com
Phone 660220907
Organization name UKRI
Department London Institute of Medical Sciences
Street address Du Cane Road
City London
State/province London
ZIP/Postal code W12 0NN
Country United Kingdom
 
Platforms (2)
GPL19756 Illumina NextSeq 500 (Saccharomyces cerevisiae)
GPL23397 Illumina HiSeq 2500 ([Candida] glabrata; Saccharomyces cerevisiae)
Samples (27)
GSM3332088 RNA-Seq_Smc1-6HA Spt16-AID_Biological replica 1
GSM3332089 RNA-Seq_Smc1-6HA Spt16-AID_Biological replica 2
GSM3332090 RNA-Seq_Smc1-6HA_Biological replica 1
Relations
BioProject PRJNA485963
SRA SRP157912

Download family Format
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Supplementary file Size Download File type/resource
GSE118534_RAW.tar 695.7 Mb (http)(custom) TAR (of BW, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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