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| Status |
Public on Aug 06, 2021 |
| Title |
CD271 is preferentially expressed and essential for cell proliferation in squamous cell carcinoma of the lung |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Squamous cell carcinoma is a major type of cancer in the lung. While several therapeutic target molecules for lung adenocarcinoma have been identified, little is known about lung squamous cell carcinoma (LSCC). We recently reported that CD271 (p75 neurotrophin receptor) serves as a marker for tumor initiation and is a key regulator of cell proliferation in hypopharyngeal squamous cell carcinoma. In this study, we found that CD271 was also expressed in squamous cell carcinoma, but not in adenocarcinoma, of several tissues, including the lung. To examine CD271’s role in LSCC, we established xenograft cell lines from LSCC patients. Within the sorted live LSCC cell population, the CD271high cells were primarily cycling through the G2/M phase, while the CD271low cells were mostly in the G0 phase. CD271 knockdown in the LSCC cells completely suppressed their proliferation and tumor-formation capability, and increased their cell-cycle arrest in the G0 phase. In the CD271-knockdown cells, ERK-phosphorylation was decreased, while no change was observed in the IκBα-, p65-, or Akt-phosphorylation. Treatment with the MEK inhibitor U0126 decreased the LSCC cells’ proliferation capability. Microarray analysis revealed that CD271 knockdown attenuated the RAS-related pathways. The knockdown of TrkB, which forms a heterodimer with CD271 and accelerates its downstream signaling, partially inhibited the LSCC cell proliferation. These results indicated that LSCC exclusively depends on CD271 for cell proliferation, in part through ERK-signaling activation.
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| Overall design |
To compare the pathway of CD271 knockdown cells, total RNA obtained from siRNA control MCC148c (human lung squamous cell carcinoma cell line) cells (sicontrol-1 to sicontrol-4, n=4) and CD271 knockdown MCC148c cells (siCD271#1-1, siCD271#1-2, siCD271#2-1 and siCD271#2-2, n=4).
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| Contributor(s) |
Mochizuki M, Nakamura M, Sibuya R, Okazaki T, Abe J, Takahashi S, Takano A, Ito H, Yokose T, Miyagi Y, Daigo Y, Sato I, Satoh K, Sugamura K, Yamaguchi K, Tamai K |
| Citation(s) |
31019292 |
| Submission date |
Aug 06, 2018 |
| Last update date |
Nov 05, 2021 |
| Contact name |
Keiichi Tamai |
| E-mail(s) |
tamaikeiichi@med.tohoku.ac.jp
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| Organization name |
Miyagi Cancer Center
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| Street address |
1-47, Nodayama, Shiote, Medeshima
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| City |
Natori, Miyagi |
| ZIP/Postal code |
981-1293 |
| Country |
Japan |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA484690 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118141_RAW.tar |
100.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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