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Series GSE118141 Query DataSets for GSE118141
Status Public on Aug 06, 2021
Title CD271 is preferentially expressed and essential for cell proliferation in squamous cell carcinoma of the lung
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Squamous cell carcinoma is a major type of cancer in the lung. While several therapeutic target molecules for lung adenocarcinoma have been identified, little is known about lung squamous cell carcinoma (LSCC). We recently reported that CD271 (p75 neurotrophin receptor) serves as a marker for tumor initiation and is a key regulator of cell proliferation in hypopharyngeal squamous cell carcinoma. In this study, we found that CD271 was also expressed in squamous cell carcinoma, but not in adenocarcinoma, of several tissues, including the lung. To examine CD271’s role in LSCC, we established xenograft cell lines from LSCC patients. Within the sorted live LSCC cell population, the CD271high cells were primarily cycling through the G2/M phase, while the CD271low cells were mostly in the G0 phase. CD271 knockdown in the LSCC cells completely suppressed their proliferation and tumor-formation capability, and increased their cell-cycle arrest in the G0 phase. In the CD271-knockdown cells, ERK-phosphorylation was decreased, while no change was observed in the IκBα-, p65-, or Akt-phosphorylation. Treatment with the MEK inhibitor U0126 decreased the LSCC cells’ proliferation capability. Microarray analysis revealed that CD271 knockdown attenuated the RAS-related pathways. The knockdown of TrkB, which forms a heterodimer with CD271 and accelerates its downstream signaling, partially inhibited the LSCC cell proliferation. These results indicated that LSCC exclusively depends on CD271 for cell proliferation, in part through ERK-signaling activation.
Overall design To compare the pathway of CD271 knockdown cells, total RNA obtained from siRNA control MCC148c (human lung squamous cell carcinoma cell line) cells (sicontrol-1 to sicontrol-4, n=4) and CD271 knockdown MCC148c cells (siCD271#1-1, siCD271#1-2, siCD271#2-1 and siCD271#2-2, n=4).
Contributor(s) Mochizuki M, Nakamura M, Sibuya R, Okazaki T, Abe J, Takahashi S, Takano A, Ito H, Yokose T, Miyagi Y, Daigo Y, Sato I, Satoh K, Sugamura K, Yamaguchi K, Tamai K
Citation(s) 31019292
Submission date Aug 06, 2018
Last update date Nov 05, 2021
Contact name Keiichi Tamai
Organization name Miyagi Cancer Center
Street address 1-47, Nodayama, Shiote, Medeshima
City Natori, Miyagi
ZIP/Postal code 981-1293
Country Japan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (8)
GSM3319266 sicontrol-1
GSM3319267 sicontrol-2
GSM3319268 sicontrol-3
BioProject PRJNA484690

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE118141_RAW.tar 100.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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